Skip Navigation

This Article
Right arrow Full Text Freely available
Right arrow Print PDF (320K) Freely available
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Search for citing articles in:
ISI Web of Science (15)
Right arrowRequest Permissions
Right arrow Commercial Re-use Guidelines
for Open Access NAR Content
Google Scholar
Right arrow Articles by Dahan, O.
Right arrow Articles by Kupiec, M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Dahan, O.
Right arrow Articles by Kupiec, M.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Nucleic Acids Research, 2002, Vol. 30, No. 20 4361-4370
© 2002 Oxford University Press

Mutations in genes of Saccharomyces cerevisiae encoding pre-mRNA splicing factors cause cell cycle arrest through activation of the spindle checkpoint

Orna Dahan and Martin Kupiec*

Department of Molecular Microbiology and Biotechnology, Tel Aviv University, Ramat Aviv 69978, Israel

*To whom correspondence should be addressed. Tel: +972 3 640 9031; Fax: +972 3 640 9407; Email: martin{at}post.tau.ac.il

Previous work has identified a group of genes whose products play important roles in two seemingly unrelated processes: cell cycle progression and splicing. The products of these genes show a network of physical and genetic interactions suggestive of the existence of a protein complex, the cell cycle and splicing complex (CSC). Here we analyze the genetic interactions between ISY1, SYF2 and NTC20, three non-essential components of the CSC. We show that mutations in ISY1 cause lethality in the absence of Ntc20p, and that the double mutant isy1{Delta} syf2{Delta} shows a temperature-dependent cell cycle arrest. This arrest is due to lower levels of {alpha}-tubulin, a protein encoded by TUB1 and TUB3, two intron-containing genes. We show that the low levels of {alpha}-tubulin in isy1{Delta} syf2{Delta} trigger activation of the spindle checkpoint, causing cell cycle arrest. Thus, our results have uncovered an unexpected role for pre-mRNA splicing in the maintenance of the fidelity of chromosome transmission during cell division.


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 RNAHome page
R. J. Grainger, J. D. Barrass, A. Jacquier, J.-C. Rain, and J. D. Beggs
Physical and genetic interactions of yeast Cwc21p, an ortholog of human SRm300/SRRM2, suggest a role at the catalytic center of the spliceosome
RNA, December 1, 2009; 15(12): 2161 - 2173.
[Abstract] [Full Text] [PDF]

Home page
 CarcinogenesisHome page
P.-C. Chu, T.-Y. Wang, Y.-T. Lu, C.-K. Chou, Y.-C. Yang, and M.-S. Chang
Involvement of p29 in DNA damage responses and Fanconi anemia pathway
Carcinogenesis, October 1, 2009; 30(10): 1710 - 1716.
[Abstract] [Full Text] [PDF]

Home page
 RNAHome page
K.-J. Chang, H.-C. Chen, and S.-C. Cheng
Ntc90 is required for recruiting first step factor Yju2 but not for spliceosome activation
RNA, September 1, 2009; 15(9): 1729 - 1739.
[Abstract] [Full Text] [PDF]

Home page
 RNAHome page
M. Hebeisen, J. Drysdale, and R. Roy
Suppressors of the cdc-25.1(gf)-associated intestinal hyperplasia reveal important maternal roles for prp-8 and a subset of splicing factors in C. elegans
RNA, December 1, 2008; 14(12): 2618 - 2633.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
I. Kuraoka, S. Ito, T. Wada, M. Hayashida, L. Lee, M. Saijo, Y. Nakatsu, M. Matsumoto, T. Matsunaga, H. Handa, et al.
Isolation of XAB2 Complex Involved in Pre-mRNA Splicing, Transcription, and Transcription-coupled Repair
J. Biol. Chem., January 11, 2008; 283(2): 940 - 950.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
M. Shimada, C. Namikawa-Yamada, M. Nakanishi, and H. Murakami
Regulation of Cdc2p and Cdc13p Is Required for Cell Cycle Arrest Induced by Defective RNA Splicing in Fission Yeast
J. Biol. Chem., September 23, 2005; 280(38): 32640 - 32648.
[Abstract] [Full Text] [PDF]

Home page
 RNAHome page
R. J. GRAINGER and J. D. BEGGS
Prp8 protein: At the heart of the spliceosome
RNA, May 1, 2005; 11(5): 533 - 557.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. Biol.Home page
S.-W. Wang, K. Asakawa, T. Z. Win, T. Toda, and C. J. Norbury
Inactivation of the Pre-mRNA Cleavage and Polyadenylation Factor Pfs2 in Fission Yeast Causes Lethal Cell Cycle Defects
Mol. Cell. Biol., March 15, 2005; 25(6): 2288 - 2296.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
O. Dahan and M. Kupiec
The Saccharomyces cerevisiae gene CDC40/PRP17 controls cell cycle progression through splicing of the ANC1 gene
Nucleic Acids Res., May 7, 2004; 32(8): 2529 - 2540.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
G. Chawla, A. K. Sapra, U. Surana, and U. Vijayraghavan
Dependence of pre-mRNA introns on PRP17, a non-essential splicing factor: implications for efficient progression through cell cycle transitions
Nucleic Acids Res., May 1, 2003; 31(9): 2333 - 2343.
[Abstract] [Full Text] [PDF]


Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.