Skip Navigation

This Article
Right arrow Full Text Freely available
Right arrow Print PDF (221K) Freely available
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Search for citing articles in:
ISI Web of Science (23)
Right arrowRequest Permissions
Right arrow Commercial Re-use Guidelines
for Open Access NAR Content
Google Scholar
Right arrow Articles by Ray, P. S.
Right arrow Articles by Das, S.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Ray, P. S.
Right arrow Articles by Das, S.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Nucleic Acids Research, 2002, Vol. 30, No. 20 4500-4508
© 2002 Oxford University Press

La autoantigen is required for the internal ribosome entry site-mediated translation of Coxsackievirus B3 RNA

Partho Sarothi Ray and Saumitra Das*

Department of Microbiology and Cell Biology, Indian Institute of Science, Bangalore-560012, India

*To whom correspondence should be addressed. Tel: +91 080 394 2886; Fax: +91 080 360 2697; Email: sdas{at}mcbl.iisc.ernet.in

Translation initiation in Coxsackievirus B3 (CVB3) occurs via ribosome binding to an internal ribosome entry site (IRES) located in the 5'-untranslated region (UTR) of the viral RNA. This unique mechanism of translation initiation requires various trans-acting factors from the host. We show that human La autoantigen (La) binds to the CVB3 5'-UTR and also demonstrate the dose-dependent effect of exogenously added La protein in stimulating CVB3 IRES-mediated translation. The requirement of La for CVB3 IRES mediated translation has been further demonstrated by inhibition of translation as a result of sequestering La and its restoration by exogenous addition of recombinant La protein. The abundance of La protein in various mouse tissue extracts has been probed using anti-La antibody. Pancreatic tissue, a target organ for CVB3 infection, was found to have a large abundance of La protein which was demonstrated to interact with the CVB3 5'-UTR. Furthermore, exogenous addition of pancreas extract to in vitro translation reactions resulted in a dose dependent stimulation of CVB3 IRES-mediated translation. These observations indicate the role of La in CVB3 IRES-mediated translation, and suggest its possible involvement in the efficient translation of the viral RNA in the pancreas.


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 J. Virol.Home page
V. Bitko, A. Musiyenko, M. A. Bayfield, R. J. Maraia, and S. Barik
Cellular La Protein Shields Nonsegmented Negative-Strand RNA Viral Leader RNA from RIG-I and Enhances Virus Growth by Diverse Mechanisms
J. Virol., August 15, 2008; 82(16): 7977 - 7987.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. Biol.Home page
E. I. Schwartz, R. V. Intine, and R. J. Maraia
CK2 Is Responsible for Phosphorylation of Human La Protein Serine-366 and Can Modulate rpL37 5'-Terminal Oligopyrimidine mRNA Metabolism
Mol. Cell. Biol., November 1, 2004; 24(21): 9580 - 9591.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
R. Pudi, P. Srinivasan, and S. Das
La Protein Binding at the GCAC Site Near the Initiator AUG Facilitates the Ribosomal Assembly on the Hepatitis C Virus RNA to Influence Internal Ribosome Entry Site-mediated Translation
J. Biol. Chem., July 16, 2004; 279(29): 29879 - 29888.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
P. S. Ray and S. Das
Inhibition of hepatitis C virus IRES-mediated translation by small RNAs analogous to stem-loop structures of the 5'-untranslated region
Nucleic Acids Res., March 12, 2004; 32(5): 1678 - 1687.
[Abstract] [Full Text] [PDF]


Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.