Skip Navigation

This Article
Right arrow Full Text Freely available
Right arrow Print PDF (269K) Freely available
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Search for citing articles in:
ISI Web of Science (5)
Right arrowRequest Permissions
Right arrow Commercial Re-use Guidelines
for Open Access NAR Content
Google Scholar
Right arrow Articles by Shukla, G. C.
Right arrow Articles by Padgett, R. A.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Shukla, G. C.
Right arrow Articles by Padgett, R. A.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Nucleic Acids Research, 2002, Vol. 30, No. 21 4650-4657
© 2002 Oxford University Press

Domains of human U4atac snRNA required for U12-dependent splicing in vivo

Girish C. Shukla, Andrea J. Cole, Rosemary C. Dietrich and Richard A. Padgett*

Department of Molecular Biology, Lerner Research Institute, Cleveland Clinic Foundation, 9500 Euclid Avenue, Cleveland, OH 44195, USA

*To whom correspondence should be addressed. Tel: +1 216 445 2692; Fax: +1 216 444 0512; Email: padgetr{at}ccf.org

U4atac snRNA forms a base-paired complex with U6atac snRNA. Both snRNAs are required for the splicing of the minor U12-dependent class of eukaryotic nuclear introns. We have developed a new genetic suppression assay to investigate the in vivo roles of several regions of U4atac snRNA in U12-dependent splicing. We show that both the stem I and stem II regions, which have been proposed to pair with U6atac snRNA, are required for in vivo splicing. Splicing activity also requires U4atac sequences in the 5' stem–loop element that bind a 15.5 kDa protein that also binds to a similar region of U4 snRNA. In contrast, mutations in the region immediately following the stem I interaction region, as well as a deletion of the distal portion of the 3' stem–loop element, were active for splicing. Complete deletion of the 3' stem–loop element abolished in vivo splicing function as did a mutation of the Sm protein binding site. These results show that the in vivo sequence requirements of U4atac snRNA are similar to those described previously for U4 snRNA using in vitro assays and provide experimental support for models of the U4atac/U6atac snRNA interaction.


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 RNAHome page
R. C. Dietrich, R. A. Padgett, and G. C. Shukla
The conserved 3' end domain of U6atac snRNA can direct U6 snRNA to the minor spliceosome
RNA, June 1, 2009; 15(6): 1198 - 1207.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. Biol.Home page
A. Damianov, S. Schreiner, and A. Bindereif
Recycling of the U12-Type Spliceosome Requires p110, a Component of the U6atac snRNP
Mol. Cell. Biol., February 15, 2004; 24(4): 1700 - 1708.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
G. C. Shukla and R. A. Padgett
U4 small nuclear RNA can function in both the major and minor spliceosomes
PNAS, January 6, 2004; 101(1): 93 - 98.
[Abstract] [Full Text] [PDF]


Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.