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Nucleic Acids Research, 2002, Vol. 30, No. 21 4667-4674
© 2002 Oxford University Press

An analysis of the sequence requirements of EDEN-BP for specific RNA binding

Sylvie Bonnet-Corven, Yann Audic, Francis Omilli and H. Beverley Osborne*

UMR 6061 CNRS–Génétique et Développement, IFR 97, Université de Rennes 1, Faculté de Médecine, 2 Avenue Léon Bernard, CS 34317, 35043 Rennes Cedex, France

*To whom correspondence should be addressed. Tel: +33 223 23 45 23; Fax: +33 223 23 44 78; Email: beverley.osborne{at}univ-rennes1.fr

EDEN-BP (embryo deadenylation element-binding protein) binds specifically to the EDEN motif in the 3'-untranslated regions of maternal mRNAs and targets these mRNAs for deadenylation and translational repression in Xenopus laevis embryos. EDEN-BP contains three RNA recognition motifs (RRMs) and is related to the elav family of RNA-binding proteins. In the present study we show that the two N-terminal RRMs of EDEN-BP are necessary for the interaction with EDEN as well as a part of the linker region (between RRM2 and RRM3). Using a band shift assay we show that two different complexes are formed according to the size and, therefore, the functional nature of the EDEN motif. Finally, we show that EDEN-BP can form a dimer in a two-hybrid assay. Accordingly, we suggest that the functional configuration of EDEN-BP is a dimer.


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