Skip Navigation

This Article
Right arrow Full Text Freely available
Right arrow Print PDF (244K) Freely available
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Search for citing articles in:
ISI Web of Science (14)
Right arrowRequest Permissions
Right arrow Commercial Re-use Guidelines
for Open Access NAR Content
Google Scholar
Right arrow Articles by Bergeron, L. J.
Right arrow Articles by Perreault, J.-P.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Bergeron, L. J.
Right arrow Articles by Perreault, J.-P.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Nucleic Acids Research, 2002, Vol. 30, No. 21 4682-4691
© 2002 Oxford University Press

Development and comparison of procedures for the selection of delta ribozyme cleavage sites within the hepatitis B virus

Lucien Junior Bergeron and Jean-Pierre Perreault*

RNA Group/Groupe ARN, Département de Biochimie, Faculté de Médecine, Université de Sherbrooke, Sherbrooke, Québec J1H 5N4, Canada

*To whom correspondence should be addressed. Tel: +1 819 564 5310; Fax: +1 819 564 5340; Email: jperre01{at}courrier.usherb.ca

Delta ribozyme possesses several unique features related to the fact that it is the only catalytic RNA known to be naturally active in human cells. This makes it attractive as a therapeutic tool for the inactivation of clinically relevant RNAs. However, several hurdles must be overcome prior to the development of useful gene-inactivation systems based on delta ribozyme. We have developed three procedures for the selection of potential delta ribozyme target sites within the hepatitis B virus (HBV) pregenome: (i) the use of bioinformatic tools coupled to biochemical assays; (ii) RNase H hydrolysis with a pool of oligonucleotides; and (iii) cleavage assays with a pool of ribozymes. The results obtained with delta ribozyme show that these procedures are governed by several rules, some of which are different from those both for other catalytic RNAs and antisense oligonucleotides. Together, these procedures identified 12 sites in the HBV pregenome that can be cleaved by delta ribozymes, although with different efficiencies. Clearly, both target site accessibility and the ability to form an active ribozyme–substrate complex constitute interdependent factors that can best be addressed using a combinatorial library of either oligonucleotides or ribozymes.


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 Appl. Environ. Microbiol.Home page
K. Fiola, J.-P. Perreault, and B. Cousineau
Gene Targeting in the Gram-Positive Bacterium Lactococcus lactis, Using Various Delta Ribozymes
Appl. Envir. Microbiol., January 1, 2006; 72(1): 869 - 879.
[Abstract] [Full Text] [PDF]

Home page
 RNAHome page
L. J. BERGERON, C. REYMOND, and J.-P. PERREAULT
Functional characterization of the SOFA delta ribozyme
RNA, December 1, 2005; 11(12): 1858 - 1868.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
L. J. Bergeron and J.-P. Perreault
Target-dependent on/off switch increases ribozyme fidelity
Nucleic Acids Res., February 24, 2005; 33(4): 1240 - 1248.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
F. D'Anjou, L. J. Bergeron, N. B. Larbi, I. Fournier, M. Salzet, J.-P. Perreault, and R. Day
Silencing of SPC2 Expression Using an Engineered {delta} Ribozyme in the Mouse {beta}TC-3 Endocrine Cell Line
J. Biol. Chem., April 2, 2004; 279(14): 14232 - 14239.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
P. Deschenes, J. Ouellet, J. Perreault, and J.-P. Perreault
Formation of the P1.1 pseudoknot is critical for both the cleavage activity and substrate specificity of an antigenomic trans-acting hepatitis delta ribozyme
Nucleic Acids Res., April 15, 2003; 31(8): 2087 - 2096.
[Abstract] [Full Text] [PDF]


Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.