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Nucleic Acids Research, 2002, Vol. 30, No. 21 e123
© 2002 Oxford University Press

AarI, a restriction endonuclease from Arthrobacter aurescens SS2-322, which recognizes the novel non-palindromic sequence 5'-CACCTGC(N)4/8-3'

R. Grigaite, Z. Maneliene1 and A. Janulaitis*,1

Fermentas UAB and 1 Institute of Biotechnology, Graiciuno 8, 2028 Vilnius, Lithuania

*To whom correspondence should be addressed. Tel: +370 2 602110; Fax: +370 2 602116; Email: janulait{at}ibt.lt

A new type II restriction endonuclease AarI has been isolated from Arthrobacter aurescens SS2-322. AarI recognizes the non-palindromic heptanucleotide sequence 5'-CACCTGC(N)4/8-3' and makes a staggered cut at the fourth and eighth bases downstream of the target duplex producing a four base 5'-protruding end. AarI activity is stimulated by oligodeoxyribonucleotide duplexes containing an enzyme-specific recognition sequence.


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