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Nucleic Acids Research, 2002, Vol. 30, No. 23 e130
© 2002 Oxford University Press

Polyamine-assisted rapid and clean cleavage of oligonucleotides from cis-diol bearing universal support

P. Kumar, G. Dhawan, R. Chandra1 and K. C. Gupta*

Nucleic Acids Research Laboratory, Institute of Genomics and Integrative Biology (formerly Centre for Biochemical Technology), Mall Road, Delhi University Campus, Delhi 110 007, India and 1 Dr B. R. Ambedkar Center for Biomedical Research, University of Delhi, Delhi 110 007, India

*To whom correspondence should be addressed. Tel: +91 11 766 6156; Fax: +91 11 766 7471; Email: kcgupta{at}cbt.res.in
Dedicated to Prof. H. Seliger, Sektion Polymere, University of Ulm, Germany, on the occasion of his 65th birthday

Polyamine-assisted deprotection conditions have been developed for the rapid and clean cleavage of oligonucleotide chains from a cis-diol group bearing universal polymer support, making it compatible with modern oligonucleotide synthesis via all types of phosphoramidite synthons, including base labile protecting group bearing synthons as well. The synthesized oligonucleotides were found to be comparable with the corresponding standard oligomers with respect to their retention time on HPLC, mass on MALDI-TOF and biological activity in PCR amplification.


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