Skip Navigation

This Article
Right arrow Full Text Freely available
Right arrow Print PDF (148K) Freely available
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrowRequest Permissions
Right arrow Commercial Re-use Guidelines
for Open Access NAR Content
Google Scholar
Right arrow Articles by Yang, X.
Right arrow Articles by Gorenstein, D. G.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Yang, X.
Right arrow Articles by Gorenstein, D. G.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Nucleic Acids Research, 2002, Vol. 30, No. 23 e132
© 2002 Oxford University Press

Construction and selection of bead-bound combinatorial oligonucleoside phosphorothioate and phosphorodithioate aptamer libraries designed for rapid PCR-based sequencing

Xianbin Yang, Suzanne E. Bassett, Xin Li, Bruce A. Luxon, Norbert K. Herzog1, Robert E. Shope1, Judy Aronson1, Tarl W. Prow1, James F. Leary2, Romy Kirby3, Andrew D. Ellington3 and David G. Gorenstein*

Sealy Center for Structural Biology and Department of Human Biological Chemistry and Genetics, 301 University Boulevard, 1 Department of Pathology and 2 Department of Internal Medicine, The University of Texas Medical Branch at Galveston, TX 77555-1157, USA and 3 Department of Chemistry and Biochemistry, Institute for Cellular and Molecular Biology, University of Texas at Austin, TX 78712, USA

*To whom correspondence should be addressed. Tel: +1 409 747 6800; Fax: +1 409 747 6850; Email: david{at}nmr.utmb.edu

Chemically synthesized combinatorial libraries of unmodified or modified nucleic acids have not previously been used in methods to rapidly select oligonucleotides binding to target biomolecules such as proteins. Phosphorothioate oligonucleotides (S-ODNs) or phosphorodithioate oligonucleotides (S2-ODNs) with sulfurs replacing one or both of the non-bridging phosphate oxygens bind to proteins more tightly than unmodified oligonucleotides and have the potential to be used as diagnostic reagents and therapeutics. We have applied a split synthesis methodology to create one-bead one-S-ODN and one-bead one-S2-ODN libraries. Binding and selection of specific beads to the transcription factor NF-{kappa}B p50/p50 protein were demonstrated. Sequencing both the nucleic acid bases and the positions of any 3'-O-thioate/dithioate linkages was carried out by using a novel PCR-based identification tag of the selected beads. This approach allows us to rapidly and conveniently identify S-ODNs or S2-ODNs that bind to proteins.


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 J. Gen. Virol.Home page
S. M. Fennewald, E. P. Scott, L. Zhang, X. Yang, J. F. Aronson, D. G. Gorenstein, B. A. Luxon, R. E. Shope, D. W. C. Beasley, A. D. T. Barrett, et al.
Thioaptamer decoy targeting of AP-1 proteins influences cytokine expression and the outcome of arenavirus infections
J. Gen. Virol., March 1, 2007; 88(3): 981 - 990.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
T. Kojima, Y. Takei, M. Ohtsuka, Y. Kawarasaki, T. Yamane, and H. Nakano
PCR amplification from single DNA molecules on magnetic beads in emulsion: application for high-throughput screening of transcription factor targets
Nucleic Acids Res., October 6, 2005; 33(17): e150 - e150.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
X. Yang, X. Li, T. W. Prow, L. M. Reece, S. E. Bassett, B. A. Luxon, N. K. Herzog, J. Aronson, R. E. Shope, J. F. Leary, et al.
Immunofluorescence assay and flow-cytometry selection of bead-bound aptamers
Nucleic Acids Res., May 15, 2003; 31(10): e54 - e54.
[Abstract] [Full Text] [PDF]


Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.