Skip Navigation

This Article
Right arrow Full Text Freely available
Right arrow Print PDF (187K) Freely available
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Search for citing articles in:
ISI Web of Science (8)
Right arrowRequest Permissions
Right arrow Commercial Re-use Guidelines
for Open Access NAR Content
Google Scholar
Right arrow Articles by Jäger, A.
Right arrow Articles by Klug, G.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Jäger, A.
Right arrow Articles by Klug, G.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Nucleic Acids Research, 2002, Vol. 30, No. 24 5436-5443
© 2002 Oxford University Press

Individual gvp transcript segments in Haloferax mediterranei exhibit varying half-lives, which are differentially affected by salt concentration and growth phase

Andreas Jäger, Regina Samorski, Felicitas Pfeifer1 and Gabriele Klug*

Institut für Mikrobiologie und Molekularbiologie, Justus-Liebig-Universität Giessen, Heinrich-Buff-Ring 26, D-35392 Giessen, Germany and 1 Institut für Mikrobiologie und Genetik, Technische Universität Darmstadt, D-64287 Darmstadt, Germany

*To whom correspondence should be addressed. Tel: +49 641 99 355 42; Fax: +49 641 99 355 49; Email: gabriele.klug{at}mikro.bio.uni-giessen.de

The mc-gvp genes for gas vesicle formation in Haloferax mediterranei are transcribed from two promoters located in front of the mc-gvpA and mc-gvpD genes. The different transcripts originating from both promoters show different abundances dependent on salt concentration in the medium and growth phase. Here we show that the half-lives of these transcripts differ significantly and that the small gvp transcripts exhibit higher stabilities than the larger gvp transcripts. While the stability of most gvp transcripts is independent of the salt concentration in the medium, the gvpA mRNA decays about twice as fast in cultures grown at 18% salt compared to cultures grown at 25% salt. The stability of the 0.45 kb transcript population derived from the 5' part of the gvpD gene depends on the growth phase of the culture. Thus, differences in mRNA stability contribute to the salt-dependent and growth phase-dependent abundance of gvp transcripts. This implies that, like in bacteria and eukarya, mRNA processing contributes to regulated gene expression in archaea.


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 J. Bacteriol.Home page
K. L. Anderson, E. E. Apolinario, S. R. MacAuley, and K. R. Sowers
A 5' Leader Sequence Regulates Expression of Methanosarcinal CO Dehydrogenase/Acetyl Coenzyme A Synthase
J. Bacteriol., November 15, 2009; 191(22): 7123 - 7128.
[Abstract] [Full Text] [PDF]

Home page
 J. Bacteriol.Home page
S. Hundt, A. Zaigler, C. Lange, J. Soppa, and G. Klug
Global Analysis of mRNA Decay in Halobacterium salinarum NRC-1 at Single-Gene Resolution Using DNA Microarrays
J. Bacteriol., October 1, 2007; 189(19): 6936 - 6944.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
S. Wagner and G. Klug
An Archaeal Protein with Homology to the Eukaryotic Translation Initiation Factor 5A Shows Ribonucleolytic Activity
J. Biol. Chem., May 11, 2007; 282(19): 13966 - 13976.
[Abstract] [Full Text] [PDF]

Home page
 MicrobiologyHome page
J. Soppa
From genomes to function: haloarchaea as model organisms.
Microbiology, March 1, 2006; 152(Pt 3): 585 - 590.
[Abstract] [Full Text] [PDF]

Home page
 MicrobiologyHome page
A. Hofacker, K.-M. Schmitz, A. Cichonczyk, S. Sartorius-Neef, and F. Pfeifer
GvpE- and GvpD-mediated transcription regulation of the p-gvp genes encoding gas vesicles in Halobacterium salinarum
Microbiology, June 1, 2004; 150(6): 1829 - 1838.
[Abstract] [Full Text] [PDF]


Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.