Skip Navigation

This Article
Right arrow Full Text Freely available
Right arrow Print PDF (694K) Freely available
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Search for citing articles in:
ISI Web of Science (23)
Right arrowRequest Permissions
Right arrow Commercial Re-use Guidelines
for Open Access NAR Content
Google Scholar
Right arrow Articles by Lukaszewicz, M.
Right arrow Articles by Jagura-Burdzy, G.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Lukaszewicz, M.
Right arrow Articles by Jagura-Burdzy, G.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Nucleic Acids Research, 2002, Vol. 30, No. 4 1046-1055
© 2002 Oxford University Press

Functional dissection of the ParB homologue (KorB) from IncP-1 plasmid RK2

M. Lukaszewicz1,2, K. Kostelidou2,3, A. A. Bartosik1, G. D. Cooke2, C. M. Thomas2 and G. Jagura-Burdzy1,*

1The Institute of Biochemistry and Biophysics, PAS, 02-106 Warsaw, Pawinskiego 5A, Poland, 2School of Biosciences, The University of Birmingham, Edgbaston, Birmingham B15 2TT, UK and 3Department of Biochemistry, Helenic Pasteur Institute, GR11521-Athens, Greece

Active partitioning of low-copy number plasmids requires two proteins belonging to the ParA and ParB families and a cis-acting site which ParB acts upon. Active separation of clusters of plasmid molecules to the defined locations in the cell before cell division ensures stable inheritance of the plasmids. The central control operon of IncP-1 plasmids codes for regulatory proteins involved in the global transcriptional control of operons for vegetative replication, stable maintenance and conjugative transfer. Two of these proteins, IncC and KorB, also play a role in active partitioning, as the ParA and ParB homologues, respectively. Here we describe mapping the regions in KorB responsible for four of its different functions: dimerisation, DNA binding, repression of transcription and interaction with IncC. For DNA binding, amino acids E151 to T218 are essential, while repression depends not only on DNA binding but, additionally, on the adjacent region amino acids T218 to R255. The C-terminus of KorB is the main dimerisation domain but a secondary oligomerisation region is located centrally in the region from amino acid I174 to T218. Using three different methods (potentiation of transcriptional repression, potentiation of DNA binding and activation in the yeast two-hybrid system) we identify this region as also responsible for interactions with IncC. This IncC–KorB contact differs in location from the ParA–ParB/SopA–SopB interactions in P1/F but is similar to these systems in lying close to a masked oligomerisation determinant.

* To whom correspondence should be addressed. Tel: +48 22 823 71 92; Fax: +48 22 658 46 36; Email: gjburdzy{at}ibbrain.ibb.waw.pl


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 MicrobiologyHome page
A. A. Bartosik, J. Mierzejewska, C. M. Thomas, and G. Jagura-Burdzy
ParB deficiency in Pseudomonas aeruginosa destabilizes the partner protein ParA and affects a variety of physiological parameters
Microbiology, April 1, 2009; 155(4): 1080 - 1092.
[Abstract] [Full Text] [PDF]

Home page
 J. Bacteriol.Home page
K. Lasocki, A. A. Bartosik, J. Mierzejewska, C. M. Thomas, and G. Jagura-Burdzy
Deletion of the parA (soj) Homologue in Pseudomonas aeruginosa Causes ParB Instability and Affects Growth Rate, Chromosome Segregation, and Motility
J. Bacteriol., August 1, 2007; 189(15): 5762 - 5772.
[Abstract] [Full Text] [PDF]

Home page
 J. Bacteriol.Home page
M. Dmowski, I. Sitkiewicz, and P. Ceglowski
Characterization of a Novel Partition System Encoded by the {delta} and {omega} Genes from the Streptococcal Plasmid pSM19035.
J. Bacteriol., June 1, 2006; 188(12): 4362 - 4372.
[Abstract] [Full Text] [PDF]

Home page
 MicrobiologyHome page
M. Adamczyk, P. Dolowy, M. Jonczyk, C. M. Thomas, and G. Jagura-Burdzy
The kfrA gene is the first in a tricistronic operon required for survival of IncP-1 plasmid R751
Microbiology, June 1, 2006; 152(6): 1621 - 1637.
[Abstract] [Full Text] [PDF]

Home page
 Phil Trans R Soc BHome page
T. A Leonard, J. Moller-Jensen, and J. Lowe
Towards understanding the molecular basis of bacterial DNA segregation
Phil Trans R Soc B, March 29, 2005; 360(1455): 523 - 535.
[Abstract] [Full Text] [PDF]

Home page
 Appl. Environ. Microbiol.Home page
G. Rhodes, J. Parkhill, C. Bird, K. Ambrose, M. C. Jones, G. Huys, J. Swings, and R. W. Pickup
Complete Nucleotide Sequence of the Conjugative Tetracycline Resistance Plasmid pFBAOT6, a Member of a Group of IncU Plasmids with Global Ubiquity
Appl. Envir. Microbiol., December 1, 2004; 70(12): 7497 - 7510.
[Abstract] [Full Text] [PDF]

Home page
 J. Bacteriol.Home page
A. A. Bartosik, K. Lasocki, J. Mierzejewska, C. M. Thomas, and G. Jagura-Burdzy
ParB of Pseudomonas aeruginosa: Interactions with Its Partner ParA and Its Target parS and Specific Effects on Bacterial Growth
J. Bacteriol., October 15, 2004; 186(20): 6983 - 6998.
[Abstract] [Full Text] [PDF]


Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.