Nucleic Acids Research, 2002, Vol. 30, No. 5 1262-1267
© 2002 Oxford University Press
UV-induced T
C transition at a TT photoproduct site is dependent on Saccharomyces cerevisiae polymerase 
in vivo
Department of Radiation Oncology and Winship Cancer Institute, B5111, Emory University School of Medicine, 1365 B Clifton Road NE, Atlanta, GA 30322, USA
UV-induced reversion of the arg4-17 ochre allele in Saccharomyces cerevisiae is largely dependent on translesion polymerase 
(Rad30p), known to bypass cyclobutane-type TT dimers in an error-free fashion. arg4-17 locus reversion was predominantly due to T
C transition of T127, the 3' T of a TT photoproduct site. This event was at least 20-fold reduced in a rad30 deletion mutant, irrespective of the status of nucleotide excision repair. These data correlate with known properties of 6–4 TT photoproducts and in vitro characteristics of polymerase and suggest that polymerase plays an important in vivo role in inserting G opposite the 3' T of 6–4 TT photoproducts at this site. Alternatively, an unprecedented error-prone processing of cyclobutane-type photoproducts at this site by polymerase must be assumed as the critical mechanism. Whereas photoreactivation results indeed hint at the latter possibility, a possible regulatory influence of reducing the overall UV damage load on the bypass probability of non-cyclobutane-type pyrimidine dimer photoproducts should not be dismissed.
* To whom correspondence should be addressed. Tel: +1 404 778 2170; Fax: +1 404 778 3965; Email: wsiede{at}emory.edu
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