Nucleic Acids Research, 2002, Vol. 30, No. 7 1500-1511
© 2002 Oxford University Press
NMR structure of a parallel-stranded DNA duplex at atomic resolution
Department of Chemical Sciences, Tata Institute of Fundamental Research, Homi Bhabha Road, Colaba, Mumbai 400 005, India and 1National Institutes of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, MD 20892, USA
DNA dodecamers have been designed with two cytosines on each end and intervening A and T stretches, such that the oligomers have fully complementary A:T base pairs when aligned in the parallel orientation. Spectroscopic (UV, CD and IR), NMR and molecular dynamics studies have shown that oligomers having the sequences d(CCATAATTTACC) and d(CCTATTAAATCC) form a parallel-stranded duplex when dissolved at 1:1 stoichiometry in aqueous solution. This is due to the C:C+ clamps on either end and extensive mismatches in the antiparallel orientation. The structure is stable at neutral and acidic pH. At higher temperatures, the duplex melts into single strands in a highly cooperative fashion. All adenine, cytosine and thymine nucleotides adopt the anti conformation with respect to the glycosidic bond. The A:T base pairs form reverse Watson–Crick base pairs. The duplex shows base stacking and NOEs between the base protons T(H6)/A(H8) and the sugar protons (H1'/H2'/H2'') of the preceding nucleotide, as has been observed in antiparallel duplexes. However, no NOEs are observed between base protons H2/H6/H8 of sequential nucleotides, though such NOEs are observed between T(CH3) and A(H8). A three-dimensional structure of the parallel-stranded duplex at atomic resolution has been obtained using molecular dynamics simulations under NMR constraints. The simulated structures have torsional angles very similar to those found in B-DNA duplexes, but the base stacking and helicoid parameters are significantly different.
* To whom correspondence should be addressed. Tel: +91 22 215 2971; Fax: +91 22 215 2110; Email: chary{at}tifr.res.in We dedicate this paper to the memory of the late Prof. M. A. Viswamitra (1932–2001)
CiteULike 
Connotea 
Del.icio.us What's this?
This article has been cited by other articles:
|
|
 |
|
  P. S. Pallan, P. Lubini, M. Bolli, and M. Egli Backbone-base inclination as a fundamental determinant of nucleic acid self- and cross-pairing Nucleic Acids Res., October 8, 2007; 35(19): 6611 - 6624. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 W. R Taylor Transcription and translation in an RNA world Phil Trans R Soc B, October 29, 2006; 361(1474): 1751 - 1760. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S.-H. Chou, K.-H. Chin, and A. H.-J. Wang Unusual DNA duplex and hairpin motifs Nucleic Acids Res., May 15, 2003; 31(10): 2461 - 2474. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Sunami, J. Kondo, T. Kobuna, I. Hirao, K. Watanabe, K.-i. Miura, and A. Takenaka Crystal structure of d(GCGAAAGCT) containing a parallel-stranded duplex with homo base pairs and an anti-parallel duplex with Watson-Crick base pairs Nucleic Acids Res., December 1, 2002; 30(23): 5253 - 5260. [Abstract] [Full Text] [PDF]
|
|