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Nucleic Acids Research, 2002, Vol. 30, No. 8 1859-1867
© 2002 Oxford University Press

Gene expression changes in response to E2F1 activation

Jens Stanelle, Thorsten Stiewe, Carmen C. Theseling, Martin Peter and Brigitte M. Pützer*

Centre for Cancer Research and Cancer Therapy, Institute of Molecular Biology, University of Essen, Medical School, Hufelandstrasse 55, D-45122 Essen, Germany

The p16/RB/E2F regulatory pathway, which controls transit through the G1 restriction point of the cell cycle, is one of the most frequent targets of genetic alterations in human cancer. Any of these alterations results in the deregulated expression of the transcription factor E2F, one of the key mediators of cell cycle progression. Under these conditions, E2F1 also participates in the induction of apoptosis by a p53-dependent pathway, and independently of p53. Recently, we identified the p53-homolog p73 as a first direct target of p53-independent apoptosis. Here, we used a cDNA microarray to screen an inducible E2F1-expressing Saos-2 cell line for E2F1 target genes. Expression analysis by cDNA microarray and RT–PCR revealed novel E2F1 target genes involved in E2F1-regulated cellular functions such as cell cycle control, DNA replication and apoptosis. In addition, the identification of novel E2F1 target genes participating in the processes of angiogenesis, invasion and metastasis supports the view that E2F1 plays a central role in many aspects of cancer development. These results provide new insight into the role of E2F1 in tumorigenesis as a basis for the development of novel anti-cancer therapeutics.

* To whom correspondence should be addressed. Tel: +49 201 723 3158; Fax: +49 201 723 5974; Email: brigitte.puetzer{at}uni-essen.de The authors wish it to be known that, in their opinion, the first two authors should be regarded as joint First Authors


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