Nucleic Acids Research, 2002, Vol. 30, No. 9 1895-1901
© 2002 Oxford University Press
A putative RNA editing from U to C in a mouse mitochondrial transcript
1Bios Chile Ingeniería Genética S.A., Millennium Institute for Fundamental and Applied Biology and Fundación Ciencia para la Vida, Avenida Marathon 1943, Santiago, Chile and 2Laboratorio de Biología Celular y Molecular, Facultad de Ciencias de la Salud, Universidad Nacional Andrés Bello, Avenida Republica 217, Santiago, Chile
Recently, we isolated and characterized a new mouse mitochondrial RNA molecule containing the mitochondrial 16S RNA plus 121 nt joined to the 5' end of the RNA. This fragment arises from the L strand of the same gene and we have named this transcript chimeric RNA. At position 121 of the RNA there is a C, which, according to the sequence of the mitochondrial 16S RNA gene, should be a U. We hypothesized that this RNA is synthesized having a U at position 121, which is later substituted to a C by a putative editing reaction. Based on the presence of sites for the restriction endonucleases RsaI and Fnu4HI around position 121, both forms of the RNA were detected in mouse tissues. To confirm the presence of the non-edited and putative edited RNA, a fragment containing the first 154 nt of the RNA was amplified by RT–PCR and cloned. The substitution of U for C was demonstrated by sequencing these clones. In vitro transcription experiments demonstrated that the substitution of U for C is not due to artifact of amplification or cloning. Moreover, in mitochondria from testis only the non-edited form was found. This, together with other experimental evidence, demonstrated that the base substitution was not due to polymorphism of the mitochondrial 16S RNA gene. This is the first demonstration of a substitution reaction from U to C in a mammalian mitochondrial transcript.
* To whom correspondence should be addressed at: Bios Chile Ingeniería Genética S.A., Millennium Institute for Fundamental and Applied Biology and Fundación Ciencia para la Vida, Avenida Marathon 1943, Santiago, Chile. Tel: +56 2 238 1878; Fax: +56 2 239 4250; Email: lburzio{at}bioschile.cl
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