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Nucleic Acids Research, 2002, Vol. 30, No. 9 1985-1990
© 2002 Oxford University Press

Insertions in the anticodon loop of tRNA1Gln(sufG) and tRNALys promote quadruplet decoding of CAAA

Michael O’Connor*

J. W. Wilson Laboratory, Department of Molecular and Cellular Biology and Biochemistry, Brown University, Providence, RI 02912, USA

Base insertion mutations in the anticodons of two different Escherichia coli tRNAs have been isolated that allow suppression of a series of +1 frameshift mutations. Insertion of a U between positions 34 and 35 of tRNA

or addition of a G between positions 36 and 37 of tRNALys expand the anticodons of both tRNAs similarly to 3'-GUUU-5' and allow decoding of complementary 5'-CAAA-3' quadruplets. Analysis of the suppressed mRNA sequences suggests that suppression occurs by pairing of the expanded anticodons to all four bases of the complementary, quadruplet codon. The tRNA

mutants are identical to the sufG class of frameshift suppressors isolated both in Salmonella enterica serovar Typhimurium and E.coli by Kohno and Roth and previously thought to affect tRNALys.

* Tel: +1 401 863 3652; Fax: +1 401 863 1182; Email: michael_o'connor{at}brown.edu


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