Nucleic Acids Research, 2002, Vol. 30, No. 9 1997-2003
© 2002 Oxford University Press
Heterogeneity in the rate and pattern of germline mutation at individual microsatellite loci
Department of Evolutionary Biology, Evolutionary Biology Centre, Uppsala University, Norbyvägen 18D, SE-752 36 Uppsala, Sweden, 1Department of Ecology and Systematics, PO Box 17, University of Helsinki, FIN-00014 Helsinki, Finland and 2Laboratoire d’Ecologie Evolutive Parasitaire, CNRS FRE 2365, Universite Pierre et Marie Curie, Batiment A, 7eme Etage, 7 Quai St Bernard, Case 237, F-75252 Paris Cedex 5, France
There is a lack of information on how individual microsatellite loci differ with respect to their mutation properties. Such variation will have an important bearing on our understanding of the ubiquitous occurrence of simple repeat sequences in eukaryotic genomes and on deriving proper mutation models that can be incorporated into genetic distance estimates. We genotyped 
100 families of the bird barn swallow (Hirundo rustica) for two hypervariable (heterozygosity >95%) microsatellite markers: HrU6, an (AAAG)n tetranucleotide repeat, and HrU10, an (AAGAG)n pentanucleotide repeat. A total of 27 germline mutation events were documented, corresponding to mutation rates of 0.57% (HrU6) and 1.56% (HrU10). The mutation rate increased with allele size, at 0.1% per repeat unit over the observed range of allele sizes (10–100 repeat units). Single repeat unit changes dominated, with 21/27 mutations representing the gain or loss of one repeat unit. There was no clear difference in the number of gains versus losses nor was there an effect of allele size on the magnitude or direction of mutation. Unexpectedly, the mutation rate of females (maternally transmitted mutations) was 2.5–5 times higher than that of males. Contrasting these observations with mutation data from other microsatellite loci reveals differences not only in the mutation rate, but also in the magnitude, direction and effect of sex on mutation. Thus, microsatellite mutation and evolution may be viewed as a dynamic and variable process.
* To whom correspondence should be addressed. Tel: +46 18 4716460; Fax: +46 18 4716310; Email: hans.ellegren{at}ebc.uu.se
CiteULike 
Connotea 
Del.icio.us What's this?
This article has been cited by other articles:
|
|
 |
|
  L. S. Eggert, J. S. Beadell, A. McClung, C. E. McIntosh, and R. C. Fleischer Evolution of Microsatellite Loci in the Adaptive Radiation of Hawaiian Honeycreepers J. Hered., March 1, 2009; 100(2): 137 - 147. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A.-L. Raquin, F. Depaulis, A. Lambert, N. Galic, P. Brabant, and I. Goldringer Experimental Estimation of Mutation Rates in a Wheat Population With a Gene Genealogy Approach Genetics, August 1, 2008; 179(4): 2195 - 2211. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. L. Seyfert, M. E. A. Cristescu, L. Frisse, S. Schaack, W. K. Thomas, and M. Lynch The Rate and Spectrum of Microsatellite Mutation in Caenorhabditis elegans and Daphnia pulex Genetics, April 1, 2008; 178(4): 2113 - 2121. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. Malysheva, O. N. Tokarskaya, V. G. Petrosyan, F. D. Danielyan, I. S. Darevsky, and A. P. Ryskov Genomic Variation in Parthenogenetic Lizard Darevskia armeniaca: Evidence from DNA Fingerprinting Data J. Hered., April 13, 2007; (2007) esm003v2. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
O. Paun and E. Horandl Evolution of Hypervariable Microsatellites in Apomictic Polyploid Lineages of Ranunculus carpaticola: Directional Bias at Dinucleotide Loci Genetics, September 1, 2006; 174(1): 387 - 398. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
V. V. Symonds and A. M. Lloyd An Analysis of Microsatellite Loci in Arabidopsis thaliana: Mutational Dynamics and Application Genetics, November 1, 2003; 165(3): 1475 - 1488. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. Dieringer and C. Schlotterer Two Distinct Modes of Microsatellite Mutation Processes: Evidence From the Complete Genomic Sequences of Nine Species Genome Res., October 1, 2003; 13(10): 2242 - 2251. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. Leblois, A. Estoup, and F. Rousset Influence of Mutational and Sampling Factors on the Estimation of Demographic Parameters in a "Continuous" Population Under Isolation by Distance Mol. Biol. Evol., April 1, 2003; 20(4): 491 - 502. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. M. Sibly, A. Meade, N. Boxall, M. J. Wilkinson, D. W. Corne, and J. C. Whittaker The Structure of Interrupted Human AC Microsatellites Mol. Biol. Evol., March 1, 2003; 20(3): 453 - 459. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. N. Irion, A. L. Schaffer, T. R. Famula, M. L. Eggleston, S. S. Hughes, and N. C. Pedersen Analysis of Genetic Variation in 28 Dog Breed Populations With 100 Microsatellite Markers J. Hered., January 1, 2003; 94(1): 81 - 87. [Abstract] [Full Text] [PDF]
|
|