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Nucleic Acids Research, 2002, Vol. 30, No. 9 2011-2017
© 2002 Oxford University Press

Predominance of six different hexanucleotide recoding signals 3' of read-through stop codons

Lance Harrell, Ulrich Melcher* and John F. Atkins1

Department of Biochemistry and Molecular Biology, Oklahoma State University, Stillwater, OK 74078, USA and 1Department of Human Genetics, University of Utah, Salt Lake City, UT 84112-5330, USA

Redefinition of UAG, UAA and UGA to specify a standard amino acid occurs in response to recoding signals present in a minority of mRNAs. This ‘read-through’ is in competition with termination and is utilized for gene expression. One of the recoding signals known to stimulate read-through is a hexanucleotide sequence of the form CARYYA 3' adjacent to the stop codon. The present work finds that of the 91 unique viral sequences annotated as read-through, 90% had one of six of the 64 possible codons immediately 3' of the read-through stop codon. The relative efficiency of these read-through contexts in mammalian tissue culture cells has been determined using a dual luciferase fusion reporter. The relative importance of the identity of several individual nucleotides in the different hexanucleotides is complex.

* To whom correspondence should be addressed. Tel: +1 405 744 6210; Fax +1 405 744 7799; Email: umelcher{at}biochem.okstate.edu


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