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Nucleic Acids Research, 2003, Vol. 31, No. 1 106-108
© 2003 Oxford University Press

MICdb: database of prokaryotic microsatellites

Vattipally B. Sreenu, Vishwanath Alevoor, Javaregowda Nagaraju1 and Hampapathalu A. Nagarajaram*

Laboratory of Computational Biology and Bioinformaitcs Facility, ECIL Road, Nacharam, Hyderabad 500 076, India 1 Laboratory of Molecular Genetics, Centre for DNA Fingerprinting and Diagnostics (CDFD), ECIL Road, Nacharam, Hyderabad 500 076, India

*To whom correspondence should be addressed. Tel: +91 40 715 1344; Fax: +91 40 715 5610; Email: han{at}cdfd.org.in

ABSTRACT

The MICdb (Microsatellites Database) (http://www.cdfd.org.in/micas) is a comprehensive relational database of non-redundant microsatellites extracted from fully sequenced prokaryotic genomes. The current version (1.0) of the database has been compiled from 83 genomes belonging to different phylogenetic groups. This database has been linked to MICAS, the web-based Microstatellite Analysis Server. MICAS provides a user-friendly front-end to systematically extract data on microsatellite tracts from genomes. The database contains the following information pertaining to the microsatellites: the regions (coding/non-coding, if coding, their GenBank annotations) containing microsatellite tracts; the frequencies of their occurrences, the size and the number of repeating motifs; and the sequences of the tracts. MICAS also provides an interface to Autoprimer, a primer design program to automatically design primers for selected microsatellite loci.


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