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Nucleic Acids Research, 2003, Vol. 31, No. 11 2952-2962
© 2003 Oxford University Press

A novel engineered meganuclease induces homologous recombination in yeast and mammalian cells

Jean-Charles Epinat, Sylvain Arnould, Patrick Chames, Pascal Rochaix, Dominique Desfontaines, Clémence Puzin, Amélie Patin, Alexandre Zanghellini, Frédéric Pâques and Emmanuel Lacroix

CELLECTIS S.A., 28 rue du Dr Roux, 75724 Paris Cedex 15, France

*To whom correspondence should be addressed. Tel: +33 1 47 34 30 74; Fax: +33 1 45 68 84 53; Email: lacroix{at}cellectis.com

Homologous gene targeting is the ultimate tool for reverse genetics, but its use is often limited by low efficiency. In a number of recent studies, site- specific DNA double-strand breaks (DSBs) have been used to induce efficient gene targeting. Engineering highly specific, dedicated DNA endonucleases is the key to a wider usage of this technology. In this study, we present two novel, chimeric meganucleases, derived from homing endonucleases. The first one is able to induce recombination in yeast and mammalian cells, whereas the second cleaves a novel (chosen) DNA target site. These results are a first step toward the generation of custom endonucleases for the purpose of targeted genome engineering.


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