Skip Navigation

This Article
Right arrow Full Text Freely available
Right arrow Print PDF (186K) Freely available
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Search for citing articles in:
ISI Web of Science (14)
Right arrowRequest Permissions
Right arrow Commercial Re-use Guidelines
for Open Access NAR Content
Google Scholar
Right arrow Articles by Le Derout, J.
Right arrow Articles by Hajnsdorf, E.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Le Derout, J.
Right arrow Articles by Hajnsdorf, E.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Nucleic Acids Research, 2003, Vol. 31, No. 14 4017-4023
© 2003 Oxford University Press

Hfq affects the length and the frequency of short oligo(A) tails at the 3' end of Escherichia coli rpsO mRNAs

Jacques Le Derout, Marc Folichon, Federica Briani1, Gianni Dehò1, Philippe Régnier and Eliane Hajnsdorf*

UPR 9073 du CNRS, Institut de Biologie Physico-Chimique, 13 Rue Pierre et Marie Curie, 75005 Paris, France and 1 Dipartimento di Genetica e di Biologia dei Microrganismi, Università degli Studi di Milano, Via Celoria 26, 20133 Milano, Italy

*To whom correspondence should be addressed. Tel: +33 1 58 41 51 26; Fax: +33 1 58 41 50 20; Email: Eliane.Hajnsdorf{at}ibpc.fr

Polyadenylation plays an important role in RNA degradation in bacterial cells. In Escherichia coli, exoribonucleases, mostly RNase II and polynucleotide phosphorylase, antagonize the synthesis of poly(A) tails by poly(A) polymerase I (PAP I). In accordance with earlier observations showing that only a small fraction of bacterial RNA is polyadenylated, we demonstrate here that ~10% of rpsO mRNA harbors short oligo(A) tails ranging from one to five A residues in wild-type cells. We also compared the length, frequency and distribution of poly(A) tails at the 3'-end of rpsO transcripts in vivo in the presence and absence of Hfq, a host factor that in vitro stimulates the activity of PAP I, and found that Hfq affects all three parameters. In the hfq+ strain the average length of oligo(A) tails and frequency of polyadenylated transcripts was higher than in the hfq– strain and a smaller proportion of tails was found at the 3' end of transcripts terminated at the Rho- independent terminator. Our data led us to the conclusion that Hfq is involved in the recognition of 3' RNA extremities by PAP I.


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 Nucleic Acids ResHome page
B. Reichenbach, A. Maes, F. Kalamorz, E. Hajnsdorf, and B. Gorke
The small RNA GlmY acts upstream of the sRNA GlmZ in the activation of glmS expression and is subject to regulation by polyadenylation in Escherichia coli
Nucleic Acids Res., May 1, 2008; 36(8): 2570 - 2580.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
K. Ziolkowska, P. Derreumaux, M. Folichon, O. Pellegrini, P. Regnier, I. V. Boni, and E. Hajnsdorf
Hfq variant with altered RNA binding functions
Nucleic Acids Res., January 30, 2006; 34(2): 709 - 720.
[Abstract] [Full Text] [PDF]

Home page
 J. Bacteriol.Home page
T. Aiso, H. Yoshida, A. Wada, and R. Ohki
Modulation of mRNA Stability Participates in Stationary-Phase-Specific Expression of Ribosome Modulation Factor
J. Bacteriol., March 15, 2005; 187(6): 1951 - 1958.
[Abstract] [Full Text] [PDF]

Home page
 RNAHome page
K. E. BAKER and C. CONDON
Under the Tucson sun: A meeting in the desert on mRNA decay
RNA, November 18, 2004; 10(11): 1680 - 1691.
[Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
R. Tomecki, A. Dmochowska, K. Gewartowski, A. Dziembowski, and P. P. Stepien
Identification of a novel human nuclear-encoded mitochondrial poly(A) polymerase
Nucleic Acids Res., November 16, 2004; 32(20): 6001 - 6014.
[Abstract] [Full Text] [PDF]

Home page
 J. Bacteriol.Home page
B. Sohlberg, J. Huang, and S. N. Cohen
The Streptomyces coelicolor Polynucleotide Phosphorylase Homologue, and Not the Putative Poly(A) Polymerase, Can Polyadenylate RNA
J. Bacteriol., December 15, 2003; 185(24): 7273 - 7278.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
M. Folichon, V. Arluison, O. Pellegrini, E. Huntzinger, P. Regnier, and E. Hajnsdorf
The poly(A) binding protein Hfq protects RNA from RNase E and exoribonucleolytic degradation
Nucleic Acids Res., December 15, 2003; 31(24): 7302 - 7310.
[Abstract] [Full Text] [PDF]


Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.