Skip Navigation

This Article
Right arrow Full Text Freely available
Right arrow Print PDF (212K) Freely available
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Search for citing articles in:
ISI Web of Science (50)
Right arrowRequest Permissions
Right arrow Commercial Re-use Guidelines
for Open Access NAR Content
Google Scholar
Right arrow Articles by Grigoriev, A.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Grigoriev, A.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Nucleic Acids Research, 2003, Vol. 31, No. 14 4157-4161
© 2003 Oxford University Press

On the number of protein–protein interactions in the yeast proteome

Andrei Grigoriev

GPC Biotech, Fraunhoferstrasse 20, Martinsried 82152, Germany

Tel: +49 89 85652644; Fax: +49 89 85652610; Email: andrei.grigoriev{at}gpc-biotech.com

Using two different approaches, we estimated that on average there are about five interacting partners per protein in the proteome of the yeast Saccharomyces cerevisiae. In the first approach, we used a novel method to model sampling overlap by a Bernoulli process, compared the results of two independent yeast two-hybrid interaction screens and tested the robustness of the estimate. The most stable estimate of five interactors per protein was obtained when the three most highly connected nodes in the protein interaction network were removed from the analysis (eight interactors per protein if those nodes were kept). In the second approach, we analysed a published high-confidence subset of putative interaction data obtained from multiple sources, including large-scale two-hybrid screens, complex purifications, synthetic lethals, correlated gene expression, computational predictions and previous annotations. Strikingly, the estimate was again five interactors per protein. These estimates suggest a range of ~16 000–26 000 different interaction pairs in the yeast, excluding homotypic interactions. We also discuss the approaches to estimating the rate of homotypic interactions.


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 Genome ResHome page
A. Alexeyenko and E. L.L. Sonnhammer
Global networks of functional coupling in eukaryotes from comprehensive data integration
Genome Res., June 1, 2009; 19(6): 1107 - 1116.
[Abstract] [Full Text] [PDF]

Home page
 Mol PlantHome page
H. Buschmann and C. W. Lloyd
Arabidopsis Mutants and the Network of Microtubule-Associated Functions
Mol Plant, November 1, 2008; 1(6): 888 - 898.
[Abstract] [Full Text] [PDF]

Home page
 ScienceHome page
H. Yu, P. Braun, M. A. Yildirim, I. Lemmens, K. Venkatesan, J. Sahalie, T. Hirozane-Kishikawa, F. Gebreab, N. Li, N. Simonis, et al.
High-Quality Binary Protein Interaction Map of the Yeast Interactome Network
Science, October 3, 2008; 322(5898): 104 - 110.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
M. P. H. Stumpf, T. Thorne, E. de Silva, R. Stewart, H. J. An, M. Lappe, and C. Wiuf
From the Cover: Estimating the size of the human interactome
PNAS, May 13, 2008; 105(19): 6959 - 6964.
[Abstract] [Full Text] [PDF]

Home page
 Plant CellHome page
J. W.A. Graham, T. C.R. Williams, M. Morgan, A. R. Fernie, R. G. Ratcliffe, and L. J. Sweetlove
Glycolytic Enzymes Associate Dynamically with Mitochondria in Response to Respiratory Demand and Support Substrate Channeling
PLANT CELL, November 1, 2007; 19(11): 3723 - 3738.
[Abstract] [Full Text] [PDF]

Home page
 Hum Mol GenetHome page
T. L. Karr
Fruit flies and the sperm proteome
Hum. Mol. Genet., October 15, 2007; 16(R2): R124 - R133.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
L. Hakes, S. C. Lovell, S. G. Oliver, and D. L. Robertson
Specificity in protein interactions and its relationship with sequence diversity and coevolution
PNAS, May 8, 2007; 104(19): 7999 - 8004.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
E. Sprinzak, Y. Altuvia, and H. Margalit
Colloquium Papers: Characterization and prediction of protein-protein interactions within and between complexes
PNAS, October 3, 2006; 103(40): 14718 - 14723.
[Abstract] [Full Text] [PDF]

Home page
 BioinformaticsHome page
H. Li, J. Li, and L. Wong
Discovering motif pairs at interaction sites from protein sequences on a proteome-wide scale
Bioinformatics, April 15, 2006; 22(8): 989 - 996.
[Abstract] [Full Text] [PDF]

Home page
 Hum Mol GenetHome page
M. E. Cusick, N. Klitgord, M. Vidal, and D. E. Hill
Interactome: gateway into systems biology
Hum. Mol. Genet., October 15, 2005; 14(suppl_2): R171 - R181.
[Abstract] [Full Text] [PDF]

Home page
 Genome ResHome page
E. Formstecher, S. Aresta, V. Collura, A. Hamburger, A. Meil, A. Trehin, C. Reverdy, V. Betin, S. Maire, C. Brun, et al.
Protein interaction mapping: A Drosophila case study
Genome Res., March 1, 2005; 15(3): 376 - 384.
[Abstract] [Full Text] [PDF]


Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.