Nucleic Acids Research, 2003, Vol. 31, No. 15 4332-4344
© 2003 Oxford University Press
Fep1 represses expression of the fission yeast Schizosaccharomyces pombe siderophore-iron transport system
Département de Biochimie, Université de Sherbrooke, Sherbrooke, Quebec J1H 5N4, Canada and 1 Liver Disease Section, NIDDK, National Institutes of Health, Bethesda, MD 20892-1800, USA
*To whom correspondence should be addressed. Tel: +1 819 564 5281; Fax: +1 819 564 5340; Email: simon.labbe{at}usherbrooke.ca
When iron repletes, Schizosaccharomyces pombe cells repress transcription of genes encoding components involved in the reductive iron transport system. Fep1 mediates this transcriptional control by interacting specifically with GATA-type cis-acting elements. To further investigate the role that Fep1 plays in iron homeostasis, we searched for additional Fep1-regulated genes. We found that str1+ is subject to negative transcriptional regulation, which is exerted through binding of Fep1 to a single GATA element in the str1+ promoter. Introduction of str1+ into a Saccharomyces cerevisiae fet3
arn1-4 strain led to assimilation of iron from ferrichrome, revealing that Str1 functions as a siderophore-iron transporter in S.pombe. We also identified two additional target genes of Fep1, named str2+ and str3+. We demonstrate that the str1+, str2+ and str3+ genes share a common promoter element, 5'-(A/T)GATAA-3'. We found that the N-terminal 241 residue segment of Fep1 expressed in Escherichia coli specifically interacts with the 5'-(A/T)GATAA-3' element present in each of these promoters. Consistent with this, constitutive high level str1+, str2+ and str3+ gene expression was observed in a fep1 mutant strain. Taken together, these results demonstrate that Fep1 occupies a central role in coordinating transcriptional regulation of genes encoding components of the reductive and non-reductive iron transport systems in fission yeast.
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