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Nucleic Acids Research, 2003, Vol. 31, No. 15 4481-4489
© 2003 Oxford University Press

Double-stranded RNA-mediated gene silencing in fission yeast

Mitch Raponi1,2 and Greg M. Arndt*,2

1 Department of Biochemistry and Molecular Genetics, University of New South Wales, Sydney, NSW 2052, Australia and 2 Johnson and Johnson Research Pty Ltd, 1 Central Avenue, Eveleigh, NSW 1430, Australia

*To whom correspondence should be addressed at Johnson and Johnson Research Pty Ltd, Locked Bag 4555, Strawberry Hills, NSW 2012, Australia. Tel: +61 2 8396 5837; Fax: +61 2 8396 5811; Email: garndt{at}medau.jnj.com
Present address:
Mitch Raponi, Molecular Diagnostics, Advanced Diagnostic and Cellular Systems, Ortho-Clinical Diagnostics, 3210 Merryfield Row, San Diego, CA 92121, USA

Double-stranded RNA (dsRNA) can specifically inhibit gene expression in a variety of organisms by invoking post-transcriptional degradation of homologous mRNA. Here we show that dsRNA-mediated gene regulation also occurs in the fission yeast Schizosaccharomyces pombe. We present evidence that: (i) reporter gene silencing is significantly enhanced when additional non-coding sense RNA is co-expressed with antisense RNA; (ii) expression of a panhandle RNA also silences target gene expression; (iii) expression of dsRNA is associated with siRNAs; (iv) a novel host-encoded factor which enhances antisense RNA gene silencing also enhances panhandle RNA-mediated gene inhibition. Both the exogenously introduced lacZ and c-myc genes are shown to be susceptible to dsRNA- mediated gene silencing in this model. Taken together, these data indicate that RNA-mediated gene silencing can occur through a RNAi-like mechanism in fission yeast.


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