Skip Navigation

This Article
Right arrow Full Text Freely available
Right arrow Print PDF (261K) Freely available
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Search for citing articles in:
ISI Web of Science (13)
Right arrowRequest Permissions
Right arrow Commercial Re-use Guidelines
for Open Access NAR Content
Google Scholar
Right arrow Articles by Torigoe, T.
Right arrow Articles by Kohno, K.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Torigoe, T.
Right arrow Articles by Kohno, K.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Nucleic Acids Research, 2003, Vol. 31, No. 15 4523-4530
© 2003 Oxford University Press

Low pH enhances Sp1 DNA binding activity and interaction with TBP

Takayuki Torigoe1,2, Hiroto Izumi1, Yoichiro Yoshida1, Hiroshi Ishiguchi1, Takashi Okamoto3, Hideaki Itoh2 and Kimitoshi Kohno*,1

1 Department of Molecular Biology and 2 Department of Surgery I, University of Occupational and Environmental Health, School of Medicine, 1-1 Iseigaoka, Yahatanishi-ku, Kitakyushu 807-8555, Japan and 3 Department of Molecular Genetics, Nagoya City University Medical School, Nagoya 467-8601, Japan

*To whom correspondence should be addressed. Tel: +81 93 691 7423; Fax: +81 93 692 2766; Email: k-kohno{at}med.uoeh-u.ac.jp

Sp1 is involved in the regulation of a wide variety of genes, including housekeeping genes and genes involved in tumor growth. Sp1 is a member of the C2-H2 zinc-finger family and is important for protection against cellular acidosis in cells that grow under hypoxic or acidic conditions, such as tumor cells. To obtain an insight into the molecular mechanisms underlying pH-dependent transcription by Sp1, both its DNA binding activity and its interaction with TATA binding protein (TBP) were investigated under various pH conditions. We show here that the DNA binding activity of Sp1 increased and Sp1 formed a stable interaction with TBP at low pH. These findings indicate that pH changes significantly modulate the activity of Sp1 and thus contribute to the cellular response under hypoxic or acidic conditions.


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 Hum Mol GenetHome page
A. G. Shah, M. J. Friedman, S. Huang, M. Roberts, X.-J. Li, and S. Li
Transcriptional dysregulation of TrkA associates with neurodegeneration in spinocerebellar ataxia type 17
Hum. Mol. Genet., November 1, 2009; 18(21): 4141 - 4152.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Renal Physiol.Home page
H. Memetimin, Y. Izumi, Y. Nakayama, Y. Kohda, H. Inoue, H. Nonoguchi, and K. Tomita
Low pH stimulates vasopressin V2 receptor promoter activity and enhances downregulation induced by V1a receptor stimulation
Am J Physiol Renal Physiol, September 1, 2009; 297(3): F620 - F628.
[Abstract] [Full Text] [PDF]

Home page
 Mol Cancer ResHome page
R. Cairns, I. Papandreou, and N. Denko
Overcoming Physiologic Barriers to Cancer Treatment by Molecularly Targeting the Tumor Microenvironment
Mol. Cancer Res., February 1, 2006; 4(2): 61 - 70.
[Abstract] [Full Text] [PDF]

Home page
 CarcinogenesisHome page
S. P. Duggan, W. M. Gallagher, E. J.P. Fox, M. M. Abdel-Latif, J. V. Reynolds, and D. Kelleher
Low pH induces co-ordinate regulation of gene expression in oesophageal cells
Carcinogenesis, February 1, 2006; 27(2): 319 - 327.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
T. Torigoe, H. Izumi, T. Wakasugi, I. Niina, T. Igarashi, T. Yoshida, I. Shibuya, K. Chijiiwa, K.-i. Matsuo, H. Itoh, et al.
DNA Topoisomerase II Poison TAS-103 Transactivates GC-Box-dependent Transcription via Acetylation of Sp1
J. Biol. Chem., January 14, 2005; 280(2): 1179 - 1185.
[Abstract] [Full Text] [PDF]

Home page
 Cardiovasc ResHome page
S. K Jimenez, F. Sheikh, Y. Jin, K. A Detillieux, J. Dhaliwal, E. Kardami, and P. A Cattini
Transcriptional regulation of FGF-2 gene expression in cardiac myocytes
Cardiovasc Res, June 1, 2004; 62(3): 548 - 557.
[Abstract] [Full Text] [PDF]


Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.