Skip Navigation

This Article
Right arrow Full Text Freely available
Right arrow Print PDF (263K) Freely available
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Search for citing articles in:
ISI Web of Science (15)
Right arrowRequest Permissions
Right arrow Commercial Re-use Guidelines
for Open Access NAR Content
Google Scholar
Right arrow Articles by Mitsuzawa, H.
Right arrow Articles by Ishihama, A.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Mitsuzawa, H.
Right arrow Articles by Ishihama, A.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Nucleic Acids Research, 2003, Vol. 31, No. 16 4696-4701
© 2003 Oxford University Press

Rpb7 subunit of RNA polymerase II interacts with an RNA-binding protein involved in processing of transcripts

Hiroshi Mitsuzawa*, Emi Kanda and Akira Ishihama

Department of Molecular Genetics, National Institute of Genetics, Mishima, Shizuoka 411-8540, Japan

*To whom correspondence should be addressed. Tel: +81 55 981 6741; Fax: +81 55 981 6746; Email: hmitsuza{at}lab.nig.ac.jp
Present address:
Emi Kanda and Akira Ishihama, Nippon Institute for Biological Science, Ome, Tokyo 198-0024, Japan

Rpb4–Rpb7, a dissociable subcomplex of RNA polymerase II (pol II), is required for transcription initiation. To understand the role of Rpb7 in transcription initiation or other processes in transcription, we carried out a two-hybrid screen for proteins that interact with Rpb7 of the fission yeast Schizosaccharomyces pombe. The screen identified the S.pombe homolog of the Saccharomyces cerevisiae Nrd1, an RNA-binding protein implicated in 3' end formation of small nucleolar and small nuclear RNAs transcribed by pol II. The S.pombe protein, named Seb1 for seven binding, was essential for cell viability, and bound directly to Rpb7 in vitro. Saccharomyces cerevisiae Rpb7 also interacted with Nrd1, indicating that the interaction is conserved in evolution. Glu166 and/or Asp167 of S.pombe Rpb7, residues near the C-terminus of the 172 amino acid protein, were found to be important for its interaction with Seb1. Our results suggest that Rpb7 may function to anchor a processing factor to the pol II apparatus, thereby coupling RNA processing to transcription. The role for Rpb7 is consistent with its location in the pol II complex determined by recent structural studies.


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 J. Biol. Chem.Home page
B. J. Natalizio, N. D. Robson-Dixon, and M. A. Garcia-Blanco
The Carboxyl-terminal Domain of RNA Polymerase II Is Not Sufficient to Enhance the Efficiency of Pre-mRNA Capping or Splicing in the Context of a Different Polymerase
J. Biol. Chem., March 27, 2009; 284(13): 8692 - 8702.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
A. J. Jasiak, H. Hartmann, E. Karakasili, M. Kalocsay, A. Flatley, E. Kremmer, K. Strasser, D. E. Martin, J. Soding, and P. Cramer
Genome-associated RNA Polymerase II Includes the Dissociable Rpb4/7 Subcomplex
J. Biol. Chem., September 26, 2008; 283(39): 26423 - 26427.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. Biol.Home page
V. M. Runner, V. Podolny, and S. Buratowski
The Rpb4 Subunit of RNA Polymerase II Contributes to Cotranscriptional Recruitment of 3' Processing Factors
Mol. Cell. Biol., March 15, 2008; 28(6): 1883 - 1891.
[Abstract] [Full Text] [PDF]

Home page
 JCBHome page
R. Lotan, V. Goler-Baron, L. Duek, G. Haimovich, and M. Choder
The Rpb7p subunit of yeast RNA polymerase II plays roles in the two major cytoplasmic mRNA decay mechanisms
J. Cell Biol., September 24, 2007; 178(7): 1133 - 1143.
[Abstract] [Full Text] [PDF]

Home page
 Genes Dev.Home page
R. Lotan, V. G. Bar-On, L. Harel-Sharvit, L. Duek, D. Melamed, and M. Choder
The RNA polymerase II subunit Rpb4p mediates decay of a specific class of mRNAs
Genes & Dev., December 15, 2005; 19(24): 3004 - 3016.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
H. Meka, F. Werner, S. C. Cordell, S. Onesti, and P. Brick
Crystal structure and RNA binding of the Rpb4/Rpb7 subunits of human RNA polymerase II
Nucleic Acids Res., November 10, 2005; 33(19): 6435 - 6444.
[Abstract] [Full Text] [PDF]

Home page
 Genes Dev.Home page
I. Djupedal, M. Portoso, H. Spahr, C. Bonilla, C. M. Gustafsson, R. C. Allshire, and K. Ekwall
RNA Pol II subunit Rpb7 promotes centromeric transcription and RNAi-directed chromatin silencing
Genes & Dev., October 1, 2005; 19(19): 2301 - 2306.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
S. R. Singh, N. Rekha, B. Pillai, V. Singh, A. Naorem, V. Sampath, N. Srinivasan, and P. P. Sadhale
Domainal organization of the lower eukaryotic homologs of the yeast RNA polymerase II core subunit Rpb7 reflects functional conservation
Nucleic Acids Res., January 2, 2004; 32(1): 201 - 210.
[Abstract] [Full Text] [PDF]


Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.