Skip Navigation

This Article
Right arrow Full Text Freely available
Right arrow Print PDF (395K) Freely available
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Search for citing articles in:
ISI Web of Science (31)
Right arrowRequest Permissions
Right arrow Commercial Re-use Guidelines
for Open Access NAR Content
Google Scholar
Right arrow Articles by Yang, N.
Right arrow Articles by Kazazian, H. H.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Yang, N.
Right arrow Articles by Kazazian, H. H., Jr
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Nucleic Acids Research, 2003, Vol. 31, No. 16 4929-4940
© 2003 Oxford University Press

An important role for RUNX3 in human L1 transcription and retrotransposition

Nuo Yang, Lin Zhang1, Yue Zhang and Haig H. Kazazian Jr*

Department of Genetics, University of Pennsylvania School of Medicine, Philadelphia, PA 19104, USA and 1 Center for Research on Reproduction and Women’s Health, University of Pennsylvania, Philadelphia, PA 19104, USA

*To whom correspondence should be addressed. Tel: +1 215 898 3582; Fax: +1 215 573 7760; Email: kazazian{at}mail.med.upenn.edu

LINE-1s (long interspersed nuclear elements-1) are abundant non-LTR retrotransposons that comprise 17% of the human genome. The 5' untranslated region (5'UTR) of human L1 (L1Hs) houses a poorly understood internal promoter. Here we report that mutations at a putative runt-domain transcription factor (RUNX) site (+83 to +101) in the 5'UTR decreased L1Hs transcription and retrotransposition in cell culture-based assays. Exogenous expression of RUNX3, but not the other two RUNX family members, RUNX1 and RUNX2, increased L1Hs transcription and retrotransposition, which were otherwise decreased by siRNAs targeting RUNX3 and a dominant negative RUNX. Further more, the specific interaction between RUNX3 and its binding site was demonstrated by an electrophoretic mobility shift assay (EMSA) using an anti-RUNX3 antibody. Interestingly, RUNX3 may also regulate the antisense promoter activity of L1Hs 5'UTR via another putative RUNX site (+526 to +508), as revealed by site-directed mutations and exogenous expression of RUNX factors. Our results indicate an important role for RUNX3 in L1Hs retrotransposition as well as transcription from its 5'UTR in both sense and antisense directions, and they should contribute to our understanding of the mechanism underlying L1Hs retrotransposition and its impact on the expression of adjacent cellular genes.


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 Genome ResHome page
V. P. Belancio, D. J. Hedges, and P. Deininger
Mammalian non-LTR retrotransposons: For better or worse, in sickness and in health
Genome Res., March 1, 2008; 18(3): 343 - 358.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
H. Muckenfuss, M. Hamdorf, U. Held, M. Perkovic, J. Lower, K. Cichutek, E. Flory, G. G. Schumann, and C. Munk
APOBEC3 Proteins Inhibit Human LINE-1 Retrotransposition
J. Biol. Chem., August 4, 2006; 281(31): 22161 - 22172.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
M. d. C. Seleme, M. R. Vetter, R. Cordaux, L. Bastone, M. A. Batzer, and H. H. Kazazian Jr.
Extensive individual variation in L1 retrotransposition capability contributes to human genetic diversity
PNAS, April 25, 2006; 103(17): 6611 - 6616.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
V. P. Belancio, D. J. Hedges, and P. Deininger
LINE-1 RNA splicing and influences on mammalian gene expression
Nucleic Acids Res., March 22, 2006; 34(5): 1512 - 1521.
[Abstract] [Full Text] [PDF]

Home page
 Genome ResHome page
H. Khan, A. Smit, and S. Boissinot
Molecular evolution and tempo of amplification of human LINE-1 retrotransposons since the origin of primates
Genome Res., January 1, 2006; 16(1): 78 - 87.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
N. Yang, L. Zhang, and H. H. Kazazian Jr
L1 retrotransposon-mediated stable gene silencing
Nucleic Acids Res., March 30, 2005; 33(6): e57 - e57.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
H. S. Soifer, A. Zaragoza, M. Peyvan, M. A. Behlke, and J. J. Rossi
A potential role for RNA interference in controlling the activity of the human LINE-1 retrotransposon
Nucleic Acids Res., February 8, 2005; 33(3): 846 - 856.
[Abstract] [Full Text] [PDF]

Home page
 Genome ResHome page
L. Lavie, E. Maldener, B. Brouha, E. U. Meese, and J. Mayer
The human L1 promoter: Variable transcription initiation sites and a major impact of upstream flanking sequence on promoter activity
Genome Res., November 1, 2004; 14(11): 2253 - 2260.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
J. N. Athanikar, R. M. Badge, and J. V. Moran
A YY1-binding site is required for accurate human LINE-1 transcription initiation
Nucleic Acids Res., July 22, 2004; 32(13): 3846 - 3855.
[Abstract] [Full Text] [PDF]


Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.