Skip Navigation

This Article
Right arrow Full Text Freely available
Right arrow Print PDF (247K) Freely available
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrowRequest Permissions
Right arrow Commercial Re-use Guidelines
for Open Access NAR Content
Google Scholar
Right arrow Articles by Le Berre, V.
Right arrow Articles by François, J.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Le Berre, V.
Right arrow Articles by François, J.
Related Collections
Right arrow Microarray
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Nucleic Acids Research, 2003, Vol. 31, No. 16 e88
© 2003 Oxford University Press

Dendrimeric coating of glass slides for sensitive DNA microarrays analysis

Véronique Le Berre1,2, Emmanuelle Trévisiol1,2,3, Adilia Dagkessamanskaia1, Serguei Sokol1, Anne-Marie Caminade3, Jean Pierre Majoral3, Bernard Meunier3 and Jean François*,1,2

1 Transcriptome-Biochips Platform of Genopole Toulouse Midi-Pyrénées, 2 Centre de Bioingénierie Gilbert Durand UMR-CNRS 5504, UMR-INRA 792, Institut National des Sciences Appliquées, F-31077 Toulouse cedex 04, France and 3 Laboratoire de Chimie de Coordination du CNRS, 205 Route de Narbonne, F-31077, Toulouse cedex 04, France

*To whom correspondence should be addressed. Tel: +33 5 61 55 94 92; Fax: +33 5 61 55 94 00; Email: fran_jm@insa-tlse.fr

Successful use and reliability of microarray technology is highly dependent on several factors, including surface chemistry parameters and accessibility of cDNA targets to the DNA probes fixed onto the surface. Here, we show that functionalisation of glass slides with homemade dendrimers allow production of more sensitive and reliable DNA microarrays. The dendrimers are nanometric structures of size-controlled diameter with aldehyde function at their periphery. Covalent attachment of these spherical reactive chemical structures on amino-silanised glass slides generates a reactive ~100 Å layer onto which amino-modified DNA probes are covalently bound. This new grafting chemistry leads to the formation of uniform and homogenous spots. More over, probe concentration before spotting could be reduced from 0.2 to 0.02 mg/ml with PCR products and from 20 to 5 µM with 70mer oligonucleotides without affecting signal intensities after hybridisation with Cy3- and Cy5-labelled targets. More interestingly, while the binding capacity of captured probes on dendrimer-activated glass surface (named dendrislides) is roughly similar to other functionalised glass slides from commercial sources, detection sensitivity was 2-fold higher than with other available DNA microarrays. This detection limit was estimated to 0.1 pM of cDNA targets. Altogether, these features make dendrimer-activated slides ideal for manufacturing cost-effective DNA arrays applicable for gene expression and detection of mutations.


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 Appl. Environ. Microbiol.Home page
S.-Q. Jin, B.-C. Yin, and B.-C. Ye
Multiplexed Bead-Based Mesofluidic System for Detection of Food-Borne Pathogenic Bacteria
Appl. Envir. Microbiol., November 1, 2009; 75(21): 6647 - 6654.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
C. R. Sabanayagam and J. R. Lakowicz
Increasing the sensitivity of DNA microarrays by metal-enhanced fluorescence using surface-bound silver nanoparticles
Nucleic Acids Res., January 28, 2007; 35(2): e13 - e13.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
K. Kinoshita, K. Fujimoto, T. Yakabe, S. Saito, Y. Hamaguchi, T. Kikuchi, K. Nonaka, S. Murata, D. Masuda, W. Takada, et al.
Multiple primer extension by DNA polymerase on a novel plastic DNA array coated with a biocompatible polymer
Nucleic Acids Res., January 12, 2007; 35(1): e3 - e3.
[Abstract] [Full Text] [PDF]

Home page
 Appl. Environ. Microbiol.Home page
C. Bontemps, G. Golfier, C. Gris-Liebe, S. Carrere, L. Talini, and C. Boivin-Masson
Microarray-Based Detection and Typing of the Rhizobium Nodulation Gene nodC: Potential of DNA Arrays To Diagnose Biological Functions of Interest
Appl. Envir. Microbiol., December 1, 2005; 71(12): 8042 - 8048.
[Abstract] [Full Text] [PDF]


Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.