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Nucleic Acids Research, 2003, Vol. 31, No. 17 5048-5053
© 2003 Oxford University Press

Enzymatic repair of an expanded genetic information system

Michael J. Moser and James R. Prudent*

Eragen Biosciences, Inc., 918 Deming Way, Madison, WI 53717-1944, USA

*To whom correspondence should be addressed. Tel: +1 6086629000; Fax: +1 6086629004; Email: jprudent{at}eragen.com

The excision repair machinery of a thermophilic bacterium has been shown to recognize and repair an expanded genetic base pair. Native Thermus aquaticus DNA polymerase will remove a mispaired natural base and replace it with a non-natural base to form an expanded base pair. In addition, DNA ligase will recognize a nick formed by polymerase between two non-natural base pairs and covalently attach the two strands, thus demonstrating complete repair of a bifurcated base-paired model duplex. These results add evidence to the idea that the cellular replication and repair machinery of an organism containing an expanded genetic alphabet could recognize and properly repair a site containing consecutive unnatural bases.


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