Skip Navigation

This Article
Right arrow Full Text Freely available
Right arrow Print PDF (195K) Freely available
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrowRequest Permissions
Right arrow Commercial Re-use Guidelines
for Open Access NAR Content
Google Scholar
Right arrow Articles by Banér, J.
Right arrow Articles by Nilsson, M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Banér, J.
Right arrow Articles by Nilsson, M.
Related Collections
Right arrow Polymorphism/mutation detection
Right arrow Microarray
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Nucleic Acids Research, 2003, Vol. 31, No. 17 e103
© 2003 Oxford University Press

Parallel gene analysis with allele-specific padlock probes and tag microarrays

Johan Banér, Anders Isaksson, Erik Waldenström1, Jonas Jarvius, Ulf Landegren* and Mats Nilsson

The Beijer Laboratory, Department of Genetics and Pathology, Rudbeck Laboratory, SE-751 85 Uppsala, Sweden and 1 Department of Medical Sciences, Uppsala University Hospital, SE-751 85 Uppsala, Sweden

*To whom correspondence should be addressed. Tel: +46 18 471 4907; Fax: +46 18 471 4808; Email: ulf.landegren{at}genpat.uu.se

Parallel, highly specific analysis methods are required to take advantage of the extensive information about DNA sequence variation and of expressed sequences. We present a scalable laboratory technique suitable to analyze numerous target sequences in multiplexed assays. Sets of padlock probes were applied to analyze single nucleotide variation directly in total genomic DNA or cDNA for parallel genotyping or gene expression analysis. All reacted probes were then co-amplified and identified by hybridization to a standard tag oligonucleotide array. The technique was illustrated by analyzing normal and pathogenic variation within the Wilson disease-related ATP7B gene, both at the level of DNA and RNA, using allele-specific padlock probes.


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 J. Clin. Microbiol.Home page
K. Kurt, A. Alderborn, M. Nilsson, B. Strommenger, W. Witte, and U. Nubel
Multiplexed Genotyping of Methicillin-Resistant Staphylococcus aureus Isolates by Use of Padlock Probes and Tag Microarrays
J. Clin. Microbiol., March 1, 2009; 47(3): 577 - 585.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
J. Goransson, C. Wahlby, M. Isaksson, W. M. Howell, J. Jarvius, and M. Nilsson
A single molecule array for digital targeted molecular analyses
Nucleic Acids Res., January 1, 2009; 37(1): e7 - e7.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
O. Ericsson, J. Jarvius, E. Schallmeiner, M. Howell, R. Y. Nong, H. Reuter, M. Hahn, J. Stenberg, M. Nilsson, and U. Landegren
A dual-tag microarray platform for high-performance nucleic acid and protein analyses
Nucleic Acids Res., May 1, 2008; 36(8): e45 - e45.
[Abstract] [Full Text] [PDF]

Home page
 J. Clin. Microbiol.Home page
P. Gyarmati, T. Conze, S. Zohari, N. LeBlanc, M. Nilsson, U. Landegren, J. Baner, and S. Belak
Simultaneous Genotyping of All Hemagglutinin and Neuraminidase Subtypes of Avian Influenza Viruses by Use of Padlock Probes
J. Clin. Microbiol., May 1, 2008; 46(5): 1747 - 1751.
[Abstract] [Full Text] [PDF]

Home page
 RNAHome page
A. Lagunavicius, Z. Kiveryte, V. Zimbaite-Ruskuliene, T. Radzvilavicius, and A. Janulaitis
Duality of polynucleotide substrates for Phi29 DNA polymerase: 3'->5' RNase activity of the enzyme
RNA, March 1, 2008; 14(3): 503 - 513.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
M. Szemes, P. Bonants, M. de Weerdt, J. Baner, U. Landegren, and C. D. Schoen
Diagnostic application of padlock probes--multiplex detection of plant pathogens using universal microarrays
Nucleic Acids Res., April 28, 2005; 33(8): e70 - e70.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
F. Dahl, M. Gullberg, J. Stenberg, U. Landegren, and M. Nilsson
Multiplex amplification enabled by selective circularization of large sets of genomic DNA fragments
Nucleic Acids Res., April 28, 2005; 33(8): e71 - e71.
[Abstract] [Full Text] [PDF]

Home page
 Clin. Chem.Home page
J. Baner, P. Marits, M. Nilsson, O. Winqvist, and U. Landegren
Analysis of T-Cell Receptor V{beta} Gene Repertoires after Immune Stimulation and in Malignancy by Use of Padlock Probes and Microarrays
Clin. Chem., April 1, 2005; 51(4): 768 - 775.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
M. H. Shapero, J. Zhang, A. Loraine, W. Liu, X. Di, G. Liu, and K. W. Jones
MARA: a novel approach for highly multiplexed locus-specific SNP genotyping using high-density DNA oligonucleotide arrays
Nucleic Acids Res., December 15, 2004; 32(22): e181 - e181.
[Abstract] [Full Text] [PDF]

Home page
 Genome ResHome page
J.-B. Fan, J. M. Yeakley, M. Bibikova, E. Chudin, E. Wickham, J. Chen, D. Doucet, P. Rigault, B. Zhang, R. Shen, et al.
A Versatile Assay for High-Throughput Gene Expression Profiling on Universal Array Matrices
Genome Res., May 1, 2004; 14(5): 878 - 885.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
F. Dahl, J. Baner, M. Gullberg, M. Mendel-Hartvig, U. Landegren, and M. Nilsson
Circle-to-circle amplification for precise and sensitive DNA analysis
PNAS, March 30, 2004; 101(13): 4548 - 4553.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
M. J. T. van Eijk, J. L. N. Broekhof, H. J. A. van der Poel, R. C. J. Hogers, H. Schneiders, J. Kamerbeek, E. Verstege, J. W. van Aart, H. Geerlings, J. B. Buntjer, et al.
SNPWaveTM: a flexible multiplexed SNP genotyping technology
Nucleic Acids Res., March 5, 2004; 32(4): e47 - e47.
[Abstract] [Full Text] [PDF]


Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.