Skip Navigation

This Article
Right arrow Full Text Freely available
Right arrow Print PDF (200K) Freely available
Right arrow Supplementary Material
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrowRequest Permissions
Right arrow Commercial Re-use Guidelines
for Open Access NAR Content
Google Scholar
Right arrow Articles by Mao, Y.
Right arrow Articles by Ouyang, Q.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Mao, Y.
Right arrow Articles by Ouyang, Q.
Related Collections
Right arrow Polymorphism/mutation detection
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Nucleic Acids Research, 2003, Vol. 31, No. 18 e108
© 2003 Oxford University Press

Studies of temperature-dependent electronic transduction on DNA hairpin loop sensor

Youdong Mao, Chunxiong Luo and Qi Ouyang*

Laboratory for Biophysics and Biotechnology, Department of Physics, Peking University, Beijing 100871, China

*To whom correspondence should be addressed. Tel: +86 10 62756943; Fax: +86 10 62751615; Email: qi{at}pku.edu.cn

A self-assembly monolayer (SAM) of hairpin DNA can be formed on a gold substrate in order to make special biosensors. Labeling the hairpin loop probes with electroactive compositions rather than a fluorophore illustrates interesting profiles of redox current versus temperature. For a biosensor interacting with perfectly complementary targets, the profile shows a characteristic plateau, which disappears when the targets have a single base variation. The plateau is split into multiple steps by tuning the hybridization temperature. We propose that the phenomena are due to hairpin loop compartmentalization. The novel characteristics lead to a thermal gradient detection method that permits perfect discrimination of a target sequence from single nucleotide mismatches.


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 Nucleic Acids ResHome page
Y. Mao, D. Liu, S. Wang, S. Luo, W. Wang, Y. Yang, Q. Ouyang, and L. Jiang
Alternating-electric-field-enhanced reversible switching of DNA nanocontainers with pH
Nucleic Acids Res., March 12, 2007; 35(5): e33 - e33.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
Y. Xiao, A. A. Lubin, B. R. Baker, K. W. Plaxco, and A. J. Heeger
Single-step electronic detection of femtomolar DNA by target-induced strand displacement in an electrode-bound duplex
PNAS, November 7, 2006; 103(45): 16677 - 16680.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
Y. Mao, C. Luo, W. Deng, G. Jin, X. Yu, Z. Zhang, Q. Ouyang, R. Chen, and D. Yu
Reversibly switchable DNA nanocompartment on surfaces
Nucleic Acids Res., October 26, 2004; 32(19): e144 - e144.
[Abstract] [Full Text] [PDF]


Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.