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Nucleic Acids Research, 2003, Vol. 31, No. 21 6221-6226
© 2003 Oxford University Press

Recognition of threosyl nucleotides by DNA and RNA polymerases

Veerle Kempeneers, Karen Vastmans, Jef Rozenski and Piet Herdewijn*

Rega Institute for Medical Research, Laboratory for Medicinal Chemistry, Minderbroedersstraat 10, B-3000 Leuven, Belgium

*To whom correspondence should be addressed. Tel: +32 16 337387; Fax: +31 16 337340; Email: piet.herdewijn{at}rega.kuleuven.ac.be

{alpha}-L-Threose nucleic acids (TNA) are potentially natural nucleic acids that could have acted as an evolutionary alternative to RNA. We determined whether DNA or RNA polymerases could recognize phosphorylated threosyl nucleosides. We found that for both the Vent (exo–) DNA polymerase and HIV reverse transcriptase Km values were increased and kcat values decreased for the incorporation of tTTP in comparison to their natural counterparts. Our results suggest that TNA may have played a role in the evolution of the DNA–RNA–protein world. Thus, TNA may be a candidate for further studies in evolutionary chemistry and biology.


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[Abstract] [Full Text] [PDF]


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