Skip Navigation

This Article
Right arrow Full Text Freely available
Right arrow Print PDF (1388K) Freely available
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Search for citing articles in:
ISI Web of Science (34)
Right arrowRequest Permissions
Right arrow Commercial Re-use Guidelines
for Open Access NAR Content
Google Scholar
Right arrow Articles by Worthey, E. A.
Right arrow Articles by Salavati, R.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Worthey, E. A.
Right arrow Articles by Salavati, R.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Nucleic Acids Research, 2003, Vol. 31, No. 22 6392-6408
© 2003 Oxford University Press

Article

Comparative analysis of editosome proteins in trypanosomatids

Elizabeth A. Worthey1,2, Achim Schnaufer1,2, I. Saira Mian3, Kenneth Stuart1,2 and Reza Salavati*,1,2

1 Seattle Biomedical Research Institute, Seattle, WA 98109, USA, 2 Department of Pathobiology, University of Washington, Seattle, WA 98195, USA and 3 Life Sciences Division, Lawrence Berkeley National Laboratory, Berkeley, CA 94720, USA

*To whom correspondence should be addressed at Seattle Biomedical Research Institute, 4 Nickerson Street, Seattle, WA 98109, USA. Tel: +1 206 284 8846; Fax: +1 206 284 0313; Email: rsalvati{at}u.washington.edu

Detailed comparisons of 16 editosome proteins from Trypanosoma brucei, Trypanosoma cruzi and Leishmania major identified protein motifs associated with catalysis and protein or nucleic acid interactions that suggest their functions in RNA editing. Five related proteins with RNase III-like motifs also contain a U1-like zinc finger and either dsRBM or Pumilio motifs. These proteins may provide the endoribonuclease function in editing. Two other related proteins, at least one of which is associated with U-specific 3' exonuclease activity, contain two putative nuclease motifs. Thus, editosomes contain a plethora of nucleases or proteins presumably derived from nucleases. Five additional related proteins, three of which have zinc fingers, each contain a motif associated with an OB fold; the TUTases have C-terminal folds reminiscent of RNA binding motifs, thus indicating the presence of numerous nucleic acid and/or protein binding domains, as do the two RNA ligases and a RNA helicase, which provide for additional catalytic steps in editing. These data indicate that trypanosomatid RNA editing is orchestrated by a variety of domains for catalysis, molecular interaction and structure. These domains are generally conserved within other protein families, but some are found in novel combinations in the editosome proteins.


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 Proc. Natl. Acad. Sci. USAHome page
F. Li, P. Ge, W. H. Hui, I. Atanasov, K. Rogers, Q. Guo, D. Osato, A. M. Falick, Z. H. Zhou, and L. Simpson
Structure of the core editing complex (L-complex) involved in uridine insertion/deletion RNA editing in trypanosomatid mitochondria
PNAS, July 28, 2009; 106(30): 12306 - 12310.
[Abstract] [Full Text] [PDF]

Home page
 RNAHome page
N. L. Ernst, B. Panicucci, J. Carnes, and K. Stuart
Differential functions of two editosome exoUases in Trypanosoma brucei
RNA, May 1, 2009; 15(5): 947 - 957.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
M. Niemann, H. Kaibel, E. Schluter, K. Weitzel, M. Brecht, and H. U. Goringer
Kinetoplastid RNA editing involves a 3' nucleotidyl phosphatase activity
Nucleic Acids Res., April 1, 2009; 37(6): 1897 - 1906.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. Biol.Home page
X. Guo, N. L. Ernst, and K. D. Stuart
The KREPA3 Zinc Finger Motifs and OB-Fold Domain Are Essential for RNA Editing and Survival of Trypanosoma brucei
Mol. Cell. Biol., November 15, 2008; 28(22): 6939 - 6953.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
M. Niemann, M. Brecht, E. Schluter, K. Weitzel, M. Zacharias, and H. U. Goringer
TbMP42 is a structure-sensitive ribonuclease that likely follows a metal ion catalysis mechanism
Nucleic Acids Res., August 1, 2008; 36(13): 4465 - 4473.
[Abstract] [Full Text] [PDF]

Home page
 RNAHome page
J. A. Law, S. F. O'Hearn, and B. Sollner-Webb
Trypanosoma brucei RNA editing protein TbMP42 (band VI) is crucial for the endonucleolytic cleavages but not the subsequent steps of U-deletion and U-insertion
RNA, June 1, 2008; 14(6): 1187 - 1200.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. Biol.Home page
V. S. Alatortsev, J. Cruz-Reyes, A. G. Zhelonkina, and B. Sollner-Webb
Trypanosoma brucei RNA Editing: Coupled Cycles of U Deletion Reveal Processive Activity of the Editing Complex
Mol. Cell. Biol., April 1, 2008; 28(7): 2437 - 2445.
[Abstract] [Full Text] [PDF]

Home page
 RNAHome page
S. Z. Tarun Jr, A. Schnaufer, N. L. Ernst, R. Proff, J. Deng, W. Hol, and K. Stuart
KREPA6 is an RNA-binding protein essential for editosome integrity and survival of Trypanosoma brucei
RNA, February 1, 2008; 14(2): 347 - 358.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. Biol.Home page
J. Carnes, J. R. Trotter, A. Peltan, M. Fleck, and K. Stuart
RNA Editing in Trypanosoma brucei Requires Three Different Editosomes
Mol. Cell. Biol., January 1, 2008; 28(1): 122 - 130.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
K. Rogers, G. Gao, and L. Simpson
Uridylate-specific 3' 5'-Exoribonucleases Involved in Uridylate-deletion RNA Editing in Trypanosomatid Mitochondria
J. Biol. Chem., October 5, 2007; 282(40): 29073 - 29080.
[Abstract] [Full Text] [PDF]

Home page
 RNAHome page
V. K. Babbarwal, M. Fleck, N. L. Ernst, A. Schnaufer, and K. Stuart
An essential role of KREPB4 in RNA editing and structural integrity of the editosome in Trypanosoma brucei
RNA, May 1, 2007; 13(5): 737 - 744.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. Biol.Home page
J. A. Law, S. O'Hearn, and B. Sollner-Webb
In Trypanosoma brucei RNA Editing, TbMP18 (Band VII) Is Critical for Editosome Integrity and for both Insertional and Deletional Cleavages
Mol. Cell. Biol., January 15, 2007; 27(2): 777 - 787.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
X. Kang, G. Gao, K. Rogers, A. M. Falick, S. Zhou, and L. Simpson
Reconstitution of full-round uridine-deletion RNA editing with three recombinant proteins
PNAS, September 19, 2006; 103(38): 13944 - 13949.
[Abstract] [Full Text] [PDF]

Home page
 RNAHome page
A. K. Panigrahi, N. L. Ernst, G. J. Domingo, M. Fleck, R. Salavati, and K. D. Stuart
Compositionally and functionally distinct editosomes in Trypanosoma brucei
RNA, June 1, 2006; 12(6): 1038 - 1049.
[Abstract] [Full Text] [PDF]

Home page
 RNAHome page
R. Salavati, N. L. Ernst, J. O'Rear, T. Gilliam, S. Tarun Jr., and K. Stuart
KREPA4, an RNA binding protein essential for editosome integrity and survival of Trypanosoma brucei
RNA, May 1, 2006; 12(5): 819 - 831.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
J. Carnes, J. R. Trotter, N. L. Ernst, A. Steinberg, and K. Stuart
An essential RNase III insertion editing endonuclease in Trypanosoma brucei
PNAS, November 15, 2005; 102(46): 16614 - 16619.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. Biol.Home page
J. A. Law, C. E. Huang, S. F. O'Hearn, and B. Sollner-Webb
In Trypanosoma brucei RNA Editing, Band II Enables Recognition Specifically at Each Step of the U Insertion Cycle
Mol. Cell. Biol., April 1, 2005; 25(7): 2785 - 2794.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
X. Kang, K. Rogers, G. Gao, A. M. Falick, S. Zhou, and L. Simpson
Reconstitution of uridine-deletion precleaved RNA editing with two recombinant enzymes
PNAS, January 25, 2005; 102(4): 1017 - 1022.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
A. Martins and S. Shuman
An RNA Ligase from Deinococcus radiodurans
J. Biol. Chem., December 3, 2004; 279(49): 50654 - 50661.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
I. Aphasizheva, R. Aphasizhev, and L. Simpson
RNA-editing Terminal Uridylyl Transferase 1: IDENTIFICATION OF FUNCTIONAL DOMAINS BY MUTATIONAL ANALYSIS
J. Biol. Chem., June 4, 2004; 279(23): 24123 - 24130.
[Abstract] [Full Text] [PDF]


Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.