Nucleic Acids Research, 2003, Vol. 31, No. 23 6741-6747
© 2003 Oxford University Press
Article |
Dynamic methylation of histone H3 at lysine 4 in transcriptional regulation by the androgen receptor
1 Department of Urology, 2 Department of Molecular Microbiology and 3 Department of Preventive Medicine, Norris Cancer Center, USC Keck School of Medicine, Los Angeles, CA 90089, USA and 4 Department of Medicine, University of Adelaide and Hanson Institute, Adelaide, SA, Australia
*To whom correspondence should be addressed. Tel: +1 323 865 0631; Fax: +1 323 865 0634; Email: coetzee{at}usc.edu
The methylation of histone H3 correlates with either gene expression or silencing depending on the residues modified. Methylated lysine 4 (H3-K4) is associated with transcription at active gene loci. Furthermore, it was reported that trimethylated but not dimethylated H3-K4 is exclusively associated with active chromatin in Saccharomyces cerevisiae. In the present study, we investigated the H3-K4 methylation at the human prostate specific antigen (PSA) locus following gene activation and repression via androgen receptor (AR). We show that ligand-induced, AR-mediated transcription was accompanied by rapid decreases in di- and trimethylated H3-K4 at the PSA enhancer and promoter. Moreover, the observed decreases in H3-K4 methylation were reversed when AR was inhibited by a specific AR antagonist, bicalutamide. In contrast to the decreases in methylation at the 5' transcriptional control regions of the PSA gene, H3-K4 methylation in the coding region steadily increased after a lag period of 
4 h. The results suggest a novel role of methylated H3-K4 in transcriptional regulation.
CiteULike 
Connotea 
Del.icio.us What's this?
This article has been cited by other articles:
|
|
 |
|
  C. Hoemme, A. Peerzada, G. Behre, Y. Wang, M. McClelland, K. Nieselt, M. Zschunke, C. Disselhoff, S. Agrawal, F. Isken, et al. Chromatin modifications induced by PML-RAR{alpha} repress critical targets in leukemogenesis as analyzed by ChIP-Chip Blood, March 1, 2008; 111(5): 2887 - 2895. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H. V. Heemers and D. J. Tindall Androgen Receptor (AR) Coregulators: A Diversity of Functions Converging on and Regulating the AR Transcriptional Complex Endocr. Rev., December 1, 2007; 28(7): 778 - 808. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. E. Sullivan, A. B. M. Reddy, K. Dietzmann, A. R. Suriano, V. P. Kocieda, M. Stewart, and M. Bhatia Epigenetic Regulation of Tumor Necrosis Factor Alpha Mol. Cell. Biol., July 15, 2007; 27(14): 5147 - 5160. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Gordon, D. G. Holt, A. Panigrahi, B. T. Wilhelm, H. Erdjument-Bromage, P. Tempst, J. Bahler, and B. R. Cairns Genome-Wide Dynamics of SAPHIRE, an Essential Complex for Gene Activation and Chromatin Boundaries Mol. Cell. Biol., June 1, 2007; 27(11): 4058 - 4069. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. Jia, H. C. Shen, M. Wantroba, O. Khalid, G. Liang, Q. Wang, E. Gentzschein, J. K. Pinski, F. Z. Stanczyk, P. A. Jones, et al. Locus-wide chromatin remodeling and enhanced androgen receptor-mediated transcription in recurrent prostate tumor cells. Mol. Cell. Biol., October 1, 2006; 26(19): 7331 - 7341. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. T. Okino, D. Pookot, L.-C. Li, H. Zhao, S. Urakami, H. Shiina, M. Igawa, and R. Dahiya Epigenetic Inactivation of the Dioxin-Responsive Cytochrome P4501A1 Gene in Human Prostate Cancer. Cancer Res., August 1, 2006; 66(15): 7420 - 7428. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. Ilagan, L. J. Zhang, J. Pottratz, K. Le, S. Salas, M. Iyer, L. Wu, S. S. Gambhir, and M. Carey Imaging androgen receptor function during flutamide treatment in the LAPC9 xenograft model Mol. Cancer Ther., November 1, 2005; 4(11): 1662 - 1669. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. Jia and G. A. Coetzee Androgen Receptor-Dependent PSA Expression in Androgen-Independent Prostate Cancer Cells Does Not Involve Androgen Receptor Occupancy of the PSA Locus Cancer Res., September 1, 2005; 65(17): 8003 - 8008. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Adachi and E. V. Rothenberg Cell-type-specific epigenetic marking of the IL2 gene at a distal cis-regulatory region in competent, nontranscribing T-cells Nucleic Acids Res., June 3, 2005; 33(10): 3200 - 3210. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H K Kinyamu, J Chen, and T K Archer Linking the ubiquitin-proteasome pathway to chromatin remodeling/modification by nuclear receptors J. Mol. Endocrinol., April 1, 2005; 34(2): 281 - 297. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. Y. Lee, C. Teyssier, B. D. Strahl, and M. R. Stallcup Role of Protein Methylation in Regulation of Transcription Endocr. Rev., April 1, 2005; 26(2): 147 - 170. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J Kim, L Jia, M R Stallcup, and G A Coetzee The role of protein kinase A pathway and cAMP responsive element-binding protein in androgen receptor-mediated transcription at the prostate-specific antigen locus J. Mol. Endocrinol., February 1, 2005; 34(1): 107 - 118. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 L.-C. Li, P. R. Carroll, and R. Dahiya Epigenetic Changes in Prostate Cancer: Implication for Diagnosis and Treatment J Natl Cancer Inst, January 19, 2005; 97(2): 103 - 115. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Z. Kang, O. A. Janne, and J. J. Palvimo Coregulator Recruitment and Histone Modifications in Transcriptional Regulation by the Androgen Receptor Mol. Endocrinol., November 1, 2004; 18(11): 2633 - 2648. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Sakamoto, J. Liu, A. Greene, M. Chen, and L. S. Weinstein Tissue-specific imprinting of the G protein Gs{alpha} is associated with tissue-specific differences in histone methylation Hum. Mol. Genet., April 15, 2004; 13(8): 819 - 828. [Abstract] [Full Text] [PDF]
|
|