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Nucleic Acids Research, 2003, Vol. 31, No. 23 6758-6769
© 2003 Oxford University Press

Article

Cleavage of DNA without loss of genetic information by incorporation of a disaccharide nucleoside

Koen Nauwelaerts, Karen Vastmans, Matheus Froeyen, Veerle Kempeneers, Jef Rozenski, Helmut Rosemeyer1, Arthur Van Aerschot, Roger Busson, Jeffrey C. Lacey, Ekaterina Efimtseva, Sergey Mikhailov, Eveline Lescrinier and Piet Herdewijn*

Rega Institute for Medical Research, Laboratory for Medicinal Chemistry, Minderbroedersstraat 10, B-3000 Leuven, Belgium and 1 Universität Osnabrück, Laboratory for Organic and Bioorganic Chemistry, Barbarastrasse 7, D-49069 Osnabrück, Germany

*To whom correspondence should be addressed. Tel: +32 16 337387; Fax: +32 16 337340; Email: Piet.Herdewijn{at}rega.kuleuven.ac.be

A ribose residue inserted between the 3'-OH of one nucleotide and the 5'-phosphate group of the next nucleotide, functions as a site-specific cleavage site within DNA. This extra ribose does not interrupt helix formation and it protects duplex DNA against cleavage by restriction enzymes. Cleavage can be obtained with periodate and all ribose fragments can be removed with sodium hydroxide. As a result of this, an intact natural oligodeoxynucleotide is obtained after ligation reaction, which means that site-specific cleavage and recovering of intact DNA occurs without loss of genetic information.


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