Skip Navigation

This Article
Right arrow Full Text Freely available
Right arrow Print PDF (329K) Freely available
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Search for citing articles in:
ISI Web of Science (15)
Right arrowRequest Permissions
Right arrow Commercial Re-use Guidelines
for Open Access NAR Content
Google Scholar
Right arrow Articles by Rascle, A.
Right arrow Articles by Lees, E.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Rascle, A.
Right arrow Articles by Lees, E.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Nucleic Acids Research, 2003, Vol. 31, No. 23 6882-6890
© 2003 Oxford University Press

Article

Chromatin acetylation and remodeling at the Cis promoter during STAT5-induced transcription

Anne Rascle* and Emma Lees

Department of Discovery Research, DNAX Research Inc., 901 California Avenue, Palo Alto, CA 94304, USA

*To whom correspondence should be addressed at present address: Biosciences Division, SRI International, 333 Ravenswood Avenue, Menlo Park, CA 94025, USA. Email: anne.rascle{at}sri.com

The signal transducer and activator of transcription STAT5 plays a major role in cytokine-induced expression of genes involved in cell proliferation and survival. Although several STAT5 partners have been identified, the molecular events taking place at the promoter level upon STAT5 recruitment have not yet been characterized in great detail. Using chromatin immunoprecipitation and accessibility assays, we characterized histone acetylation and chromatin remodeling events occurring during transcriptional activation of the endogenous murine Cis gene, a STAT5 target gene, in response to IL-3. We found that STAT5 binding in vivo is associated with low histone H3 and H4 acetylation levels in the proximity of the STAT5 binding sites. STAT5 recruitment also results in chromatin reorganization over that promoter region. These events (STAT5 binding, histone acetylation and chromatin remodeling) are not sufficient for transcriptional activation, which requires a non-histone protein deacetylase. These data reveal novel implications of STAT5 in chromatin regulation during cytokine-induced transcription, thus contributing to a better understanding of the mechanism of transcriptional activation by STAT5.


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 Nucleic Acids ResHome page
B. Basham, M. Sathe, J. Grein, T. McClanahan, A. D'Andrea, E. Lees, and A. Rascle
In vivo identification of novel STAT5 target genes
Nucleic Acids Res., June 1, 2008; 36(11): 3802 - 3818.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Endocrinol.Home page
E. B. Kabotyanski, M. Huetter, W. Xian, M. Rijnkels, and J. M. Rosen
Integration of Prolactin and Glucocorticoid Signaling at the {beta}-Casein Promoter and Enhancer by Ordered Recruitment of Specific Transcription Factors and Chromatin Modifiers
Mol. Endocrinol., October 1, 2006; 20(10): 2355 - 2368.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
E. A. Nelson, S. R. Walker, W. Li, X. S. Liu, and D. A. Frank
Identification of Human STAT5-dependent Gene Regulatory Elements Based on Interspecies Homology
J. Biol. Chem., September 8, 2006; 281(36): 26216 - 26224.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Gastrointest. Liver Physiol.Home page
M. L. Shumate, G. Yumet, T. A. Ahmed, and R. N. Cooney
Interleukin-1 inhibits the induction of insulin-like growth factor-I by growth hormone in CWSV-1 hepatocytes
Am J Physiol Gastrointest Liver Physiol, August 1, 2005; 289(2): G227 - G239.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
E. A. Nelson, S. R. Walker, J. V. Alvarez, and D. A. Frank
Isolation of Unique STAT5 Targets by Chromatin Immunoprecipitation-based Gene Identification
J. Biol. Chem., December 24, 2004; 279(52): 54724 - 54730.
[Abstract] [Full Text] [PDF]


Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.