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Nucleic Acids Research, 2003, Vol. 31, No. 4 e12
© 2003 Oxford University Press

Rapid generation of inducible mouse mutants

Jost Seibler, Branko Zevnik, Birgit Küter-Luks, Susanne Andreas, Heidrun Kern, Thomas Hennek, Anja Rode, Cornelia Heimann, Nicole Faust, Gunther Kauselmann, Michael Schoor, Rudolf Jaenisch1, Klaus Rajewsky2, Ralf Kühn and Frieder Schwenk*

ARTEMIS Pharmaceuticals GmbH, Neurather Ring 1, 51063 Cologne, Germany, 1 Whitehead Institute for Biomedical Research and Department of Biology, Massachusetts, Institute of Technology, Cambridge, MA, USA and 2 Center for Blood Research, Harvard Medical School, Boston, MA, USA

*To whom correspondence should be addressed. Tel: +49 221 9645315; Fax: +49 221 9645321; Email: f.schwenk{at}artemispharma.de

We have generated an optimized inducible recombination system for conditional gene targeting based on a Cre recombinase–steroid receptor fusion. This configuration allows efficient Cre-mediated recombination in most organs of the mouse upon induction, without detectable background activity. An ES cell line, was established that carries the inducible recombinase and a loxP-flanked lacZ reporter gene. Out of this line, completely ES cell-derived mice were efficiently produced through tetraploid blastocyst complementation, without the requirement of mouse breeding. Our findings provide a new concept allowing the generation of inducible mouse mutants within 6 months, as compared to 14 months using the current protocol.


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