Skip Navigation

This Article
Right arrow Full Text Freely available
Right arrow Print PDF (466K) Freely available
Right arrow Supplementary Material
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Search for citing articles in:
ISI Web of Science (36)
Right arrowRequest Permissions
Right arrow Commercial Re-use Guidelines
for Open Access NAR Content
Google Scholar
Right arrow Articles by Porwollik, S.
Right arrow Articles by McClelland, M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Porwollik, S.
Right arrow Articles by McClelland, M.
Related Collections
Right arrow Microarray
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Nucleic Acids Research, 2003, Vol. 31, No. 7 1869-1876
© 2003 Oxford University Press

A non-redundant microarray of genes for two related bacteria

Steffen Porwollik, Jonathan Frye, Liliana D. Florea1, Felisa Blackmer and Michael McClelland

Sidney Kimmel Cancer Center, 10835 Altman Row, San Diego, CA 92121, USA and 1 Celera/Applied Biosystems, 45 West Gude Drive, Rockville, MD 20850, USA

The authors wish it to be known that, in their opinion, the first two authors should be regarded as joint First Authors

A microarray with sequences from the annotated open reading frames (ORFs) in Salmonella enterica subspecies 1, serovar Typhimurium was supplemented with annotated chromosomal ORFs from serovar Typhi that are divergent from Typhimurium (>10% DNA sequence divergence). This non- redundant array was used to (i) measure changes in gene copy number in DNA from actively growing versus stationary Typhi and (ii) to reveal the transcriptional response of Typhi to peroxide, a stress similar to that experienced when they are phagocytosed by macrophages. In S.enterica subspecies 1, pairs of genomes differ in the presence or absence of ~10% of their genes. An array twice the size of that needed to cover all ORFs for one genome could carry close homologs of all the ORFs for 10 genomes. Non-redundant DNA arrays could be constructed for any group of closely related organisms that differ by the presence and absence of a few genes.


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 MicrobiologyHome page
J. A. Wright, S. S. Totemeyer, I. Hautefort, C. Appia-Ayme, M. Alston, V. Danino, G. K. Paterson, P. Mastroeni, N. Menager, M. Rolfe, et al.
Multiple redundant stress resistance mechanisms are induced in Salmonella enterica serovar Typhimurium in response to alteration of the intracellular environment via TLR4 signalling
Microbiology, September 1, 2009; 155(9): 2919 - 2929.
[Abstract] [Full Text] [PDF]

Home page
 Infect. Immun.Home page
K. Poirier, S. P. Faucher, M. Beland, R. Brousseau, V. Gannon, C. Martin, J. Harel, and F. Daigle
Escherichia coli O157:H7 Survives within Human Macrophages: Global Gene Expression Profile and Involvement of the Shiga Toxins
Infect. Immun., November 1, 2008; 76(11): 4814 - 4822.
[Abstract] [Full Text] [PDF]

Home page
 J. Clin. Microbiol.Home page
N. Arrach, S. Porwollik, P. Cheng, A. Cho, F. Long, S.-H. Choi, and M. McClelland
Salmonella Serovar Identification Using PCR-Based Detection of Gene Presence and Absence
J. Clin. Microbiol., August 1, 2008; 46(8): 2581 - 2589.
[Abstract] [Full Text] [PDF]

Home page
 J. Bacteriol.Home page
I. Hernandez-Lucas, A. L. Gallego-Hernandez, S. Encarnacion, M. Fernandez-Mora, A. G. Martinez-Batallar, H. Salgado, R. Oropeza, and E. Calva
The LysR-Type Transcriptional Regulator LeuO Controls Expression of Several Genes in Salmonella enterica Serovar Typhi
J. Bacteriol., March 1, 2008; 190(5): 1658 - 1670.
[Abstract] [Full Text] [PDF]

Home page
 MicrobiologyHome page
S. M. Townsend, E. Hurrell, I. Gonzalez-Gomez, J. Lowe, J. G. Frye, S. Forsythe, and J. L. Badger
Enterobacter sakazakii invades brain capillary endothelial cells, persists in human macrophages influencing cytokine secretion and induces severe brain pathology in the neonatal rat
Microbiology, October 1, 2007; 153(10): 3538 - 3547.
[Abstract] [Full Text] [PDF]

Home page
 J. Bacteriol.Home page
I. M. V. Thijs, S. C. J. De Keersmaecker, A. Fadda, K. Engelen, H. Zhao, M. McClelland, K. Marchal, and J. Vanderleyden
Delineation of the Salmonella enterica Serovar Typhimurium HilA Regulon through Genome-Wide Location and Transcript Analysis
J. Bacteriol., July 1, 2007; 189(13): 4587 - 4596.
[Abstract] [Full Text] [PDF]

Home page
 J. Bacteriol.Home page
E. A. Stohl and H. S. Seifert
Neisseria gonorrhoeae DNA Recombination and Repair Enzymes Protect against Oxidative Damage Caused by Hydrogen Peroxide
J. Bacteriol., November 1, 2006; 188(21): 7645 - 7651.
[Abstract] [Full Text] [PDF]

Home page
 J. Bacteriol.Home page
J. Frye, J. E. Karlinsey, H. R. Felise, B. Marzolf, N. Dowidar, M. McClelland, and K. T. Hughes
Identification of New Flagellar Genes of Salmonella enterica Serovar Typhimurium
J. Bacteriol., March 15, 2006; 188(6): 2233 - 2243.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
Séb. P. Faucher, S. Porwollik, C. M. Dozois, M. McClelland, and F. Daigle
Transcriptome of Salmonella enterica serovar Typhi within macrophages revealed through the selective capture of transcribed sequences
PNAS, February 7, 2006; 103(6): 1906 - 1911.
[Abstract] [Full Text] [PDF]

Home page
 Appl. Environ. Microbiol.Home page
A. A. Witney, G. L. Marsden, M. T. G. Holden, R. A. Stabler, S. E. Husain, J. K. Vass, P. D. Butcher, J. Hinds, and J. A. Lindsay
Design, Validation, and Application of a Seven-Strain Staphylococcus aureus PCR Product Microarray for Comparative Genomics
Appl. Envir. Microbiol., November 1, 2005; 71(11): 7504 - 7514.
[Abstract] [Full Text] [PDF]

Home page
 J. Bacteriol.Home page
T. Zeller, O. V. Moskvin, K. Li, G. Klug, and M. Gomelsky
Transcriptome and Physiological Responses to Hydrogen Peroxide of the Facultatively Phototrophic Bacterium Rhodobacter sphaeroides
J. Bacteriol., November 1, 2005; 187(21): 7232 - 7242.
[Abstract] [Full Text] [PDF]

Home page
 J. Bacteriol.Home page
S. Porwollik, C. A. Santiviago, P. Cheng, L. Florea, and M. McClelland
Differences in Gene Content between Salmonella enterica Serovar Enteritidis Isolates and Comparison to Closely Related Serovars Gallinarum and Dublin
J. Bacteriol., September 15, 2005; 187(18): 6545 - 6555.
[Abstract] [Full Text] [PDF]

Home page
 Appl. Environ. Microbiol.Home page
C. A. Morales, S. Porwollik, J. G. Frye, H. Kinde, M. McClelland, and J. Guard-Bouldin
Correlation of Phenotype with the Genotype of Egg-Contaminating Salmonella enterica Serovar Enteritidis
Appl. Envir. Microbiol., August 1, 2005; 71(8): 4388 - 4399.
[Abstract] [Full Text] [PDF]

Home page
 J. Bacteriol.Home page
K.-Y. Wu, G.-R. Liu, W.-Q. Liu, A. Q. Wang, S. Zhan, K. E. Sanderson, R. N. Johnston, and S.-L. Liu
The Genome of Salmonella enterica Serovar Gallinarum: Distinct Insertions/Deletions and Rare Rearrangements
J. Bacteriol., July 15, 2005; 187(14): 4720 - 4727.
[Abstract] [Full Text] [PDF]

Home page
 MicrobiologyHome page
S. M. Lai and H. L. Moual
PrpZ, a Salmonella enterica serovar Typhi serine/threonine protein phosphatase 2C with dual substrate specificity
Microbiology, April 1, 2005; 151(4): 1159 - 1167.
[Abstract] [Full Text] [PDF]

Home page
 J. Bacteriol.Home page
J. G. Frye, S. Porwollik, F. Blackmer, P. Cheng, and M. McClelland
Host Gene Expression Changes and DNA Amplification during Temperate Phage Induction
J. Bacteriol., February 15, 2005; 187(4): 1485 - 1492.
[Abstract] [Full Text] [PDF]

Home page
 J. Bacteriol.Home page
S. Porwollik, E. F. Boyd, C. Choy, P. Cheng, L. Florea, E. Proctor, and M. McClelland
Characterization of Salmonella enterica Subspecies I Genovars by Use of Microarrays
J. Bacteriol., September 1, 2004; 186(17): 5883 - 5898.
[Abstract] [Full Text] [PDF]

Home page
 J. Bacteriol.Home page
S. M. Bueno, C. A. Santiviago, A. A. Murillo, J. A. Fuentes, A. N. Trombert, P. I. Rodas, P. Youderian, and G. C. Mora
Precise Excision of the Large Pathogenicity Island, SPI7, in Salmonella enterica Serovar Typhi
J. Bacteriol., May 15, 2004; 186(10): 3202 - 3213.
[Abstract] [Full Text] [PDF]

Home page
 J. Bacteriol.Home page
S. Nair, S. Alokam, S. Kothapalli, S. Porwollik, E. Proctor, C. Choy, M. McClelland, S.-L. Liu, and K. E. Sanderson
Salmonella enterica Serovar Typhi Strains from Which SPI7, a 134-Kilobase Island with Genes for Vi Exopolysaccharide and Other Functions, Has Been Deleted
J. Bacteriol., May 15, 2004; 186(10): 3214 - 3223.
[Abstract] [Full Text] [PDF]

Home page
 Appl. Environ. Microbiol.Home page
J. Alvarez, S. Porwollik, I. Laconcha, V. Gisakis, A. B. Vivanco, I. Gonzalez, S. Echenagusia, N. Zabala, F. Blackmer, M. McClelland, et al.
Detection of a Salmonella enterica Serovar California Strain Spreading in Spanish Feed Mills and Genetic Characterization with DNA Microarrays
Appl. Envir. Microbiol., December 1, 2003; 69(12): 7531 - 7534.
[Abstract] [Full Text] [PDF]

Home page
 J. Clin. Microbiol.Home page
E. F. Boyd, S. Porwollik, F. Blackmer, and M. McClelland
Differences in Gene Content among Salmonella enterica Serovar Typhi Isolates
J. Clin. Microbiol., August 1, 2003; 41(8): 3823 - 3828.
[Abstract] [Full Text] [PDF]


Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.