Skip Navigation

This Article
Right arrow Full Text Freely available
Right arrow Print PDF (295K) Freely available
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Search for citing articles in:
ISI Web of Science (14)
Right arrowRequest Permissions
Right arrow Commercial Re-use Guidelines
for Open Access NAR Content
Google Scholar
Right arrow Articles by Cheng, C.-C.
Right arrow Articles by Chen, Y.-J.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Cheng, C.-C.
Right arrow Articles by Chen, Y.-J.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Nucleic Acids Research, 2003, Vol. 31, No. 8 2227-2233
© 2003 Oxford University Press

Mechanistic aspects of CoII(HAPP)(TFA)2 in DNA bulge-specific recognition

Chien-Chung Cheng, Wei-Chun Huang-Fu1, Kuo-Chan Hung, Po-Jui Chen1, Wen Jwu Wang and Yu-Ju Chen1

Department of Chemistry, Tamkang University, Taipei, Taiwan 251, Republic of China and 1 Institute of Chemistry, Academia Sinica, Taipei, Taiwan 11529, Republic of China

Correspondence may also be addressed to Yu-Ju Chen. Tel: +886 2 27898660; Fax: +886 2 27831237; Email: yjchen{at}chem.sinica.edu.tw

A novel octahedral complex CoII(HAPP)(TFA)2 [hexaazaphenantholine-cyclophane (HAPP), trifluoroacetate (TFA)] is a DNA bulge-specific probe with single-strand DNA cleavage activity in the presence of H2O2. This complex exhibits low affinity towards double-stranded DNA and low reactivity toward single-stranded DNA. Metal–HAPP complexes with different coordination number and ring size were synthesized and their selectivity and reactivity for DNA bulges were compared. The DNA sequence at the bulge site influences the intensity of cleavage at the bulge and the flanking sites after piperidine treatment. Cleavage specificity of CoII(HAPP)(TFA)2 was characterized extensively using scavenger reagents to quench the cleavage reaction and high-resolution polyacrylamide gel electrophoresis. In addition, 3'-phosphoglycolate cleavage products were trapped and analyzed by matrix-assisted laser desorption ionization time-of-flight mass spectrometry. These data were used to deduce that the DNA cleavage pathway for CoIIHAPP2+ in the presence of H2O2 involves 4'-H abstraction of the deoxyribose moiety.


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 Nucleic Acids ResHome page
N. W. Luedtke, J. S. Hwang, E. Nava, D. Gut, M. Kol, and Y. Tor
The DNA and RNA specificity of eilatin Ru(II) complexes as compared to eilatin and ethidium bromide
Nucleic Acids Res., October 1, 2003; 31(19): 5732 - 5740.
[Abstract] [Full Text] [PDF]


Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.