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Published online 9 January 2004

Nucleic Acids Research, 2004, Vol. 32, No. 1 255-262
© 2004 Oxford University Press

Drosophila RNase Z processes mitochondrial and nuclear pre-tRNA 3' ends in vivo

Edward B. Dubrovsky, Veronica A. Dubrovskaya, Louis Levinger1, Steffen Schiffer2 and Anita Marchfelder*,2

Department of Biology, Dartmouth College, Hanover, NH 03755, USA, 1 Department of Natural Sciences, York College/CUNY, Jamaica, NY 11451, USA and 2 Molekulare Botanik, Universität Ulm, 89069 Ulm, Germany

*To whom correspondence should be addressed. Tel: +49 731 5022658; Fax: +49 731 5022626; Email: anita.marchfelder{at}biologie.uni-ulm.de

Although correct tRNA 3' ends are crucial for protein biosynthesis, generation of mature tRNA 3' ends in eukaryotes is poorly understood and has so far only been investigated in vitro. We report here for the first time that eukaryotic tRNA 3' end maturation is catalysed by the endonuclease RNase Z in vivo. Silencing of the JhI-1 gene (RNase Z homolog) in vivo with RNAi in Drosophila S2 cultured cells causes accumulation of nuclear and mitochondrial pre-tRNAs, suggesting that JhI-1 encodes both forms of the tRNA 3' endonuclease RNase Z, and establishing its biological role in endonucleolytic tRNA 3' end processing. In addition our data show that in vivo 5' processing of nuclear and mitochondrial pre-tRNAs occurs before 3' processing.


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