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Published online 1 June 2004

Nucleic Acids Research, 2004, Vol. 32, No. 10 e79
© 2004 Oxford University Press

High-throughput polyribosome fractionation

Yipeng Wang, Steven Ringquist1, Ann H. Cho, Gaelle Rondeau and John Welsh*

Sidney Kimmel Cancer Center, 10835 Altman Row, San Diego, CA 92121, USA and 1 Department of Pediatrics, Division of Immunogenetics, Rangos Research Center, Children’s Hospital of Pittsburgh, University of Pittsburgh School of Medicine, Pittsburgh, PA 15213, USA

*To whom correspondence should be addressed. Tel: +1 858 450 5990 ext.282; Fax: +1 858 550 3998; Email: jwelsh{at}skcc.org

Received April 7, 2004 ; Revised and Accepted May 4, 2004

Polyribosome sedimentation velocity centrifugation can be used to identify differential regulation of the translation of mRNAs. However, ultracentrifugation presents practical limitations on the number of sedimentation velocity gradients that can be run simultaneously. A method for sedimentation velocity analysis of polyribosomes is presented that is based on low-speed centrifugation of sucrose gradients prepared in deep 96-well plates, the advantage of which is that hundreds of polyribosome fractionations can be performed simultaneously in a tabletop centrifuge.


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