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Nucleic Acids Research 2004 32(15):4563-4575; doi:10.1093/nar/gkh794
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Published online 24 August 2004

Nucleic Acids Research, Vol. 32 No. 15 © Oxford University Press 2004; all rights reserved

Thioredoxin can influence gene expression by affecting gyrase activity

Kuanyu Li, Cécile Pasternak, Elisabeth Härtig, Kerstin Haberzettl, Anthony Maxwell1 and Gabriele Klug*

Institut für Mikrobiologie und Molekularbiologie, University of Giessen, Heinrich-Buff-Ring 26-32, D-35392 Giessen, Germany and 1 Department of Biological Chemistry, John Innes Centre, Norwich Research Park, Colney, Norwich NR4 7UH, UK

* To whom correspondence should be addressed. Tel: +49 641 99 355 42; Fax: +49 641 99 355 49; Email: Gabriele.Klug{at}mikro.bio.uni-giessen.de
Present addresses: Cécile Pasternak, Laboratoire de Génétique des Bacteries Photosynthetiques, Centre de Génétique Moleculaire–Bat. 26, CNRS, 91198 Gif sur Yvette Cedex, France
Elisabeth Härtig, Institut für Mikrobiologie, TU Braunschweig, Spielmannstrasse 7, 38106 Braunschweig, Germany

Received April 23, 2004; Revised June 11, 2004; Accepted August 10, 2004

The expression of many genes of facultatively photosynthetic bacteria of the genus Rhodobacter is controlled by the oxygen tension. Among these are the genes of the puf and puc operons, which encode proteins of the photosynthetic apparatus. Previous results revealed that thioredoxins are involved in the regulated expression of these operons, but it remained unsolved as to the mechanisms by which thioredoxins affect puf and puc expression. Here we show that reduced TrxA of Rhodobacter capsulatus and Rhodobacter sphaeroides and oxidized TrxC of R.capsulatus interact with DNA gyrase and alter its DNA supercoiling activity. While TrxA enhances supercoiling, TrxC exerts a negative effect on this activity. Furthermore, inhibition of gyrase activity strongly reduces puf and puc expression. Our results reveal a new signaling pathway by which oxygen can affect the expression of bacterial genes.


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