Published online 15 September 2004
Nucleic Acids Research, Vol. 32 No. 16 © Oxford University Press 2004; all rights reserved
An insulator blocks spreading of histone acetylation and interferes with RNA polymerase II transfer between an enhancer and gene
Laboratory of Cellular and Developmental Biology, NIDDK, NIH, Bethesda, MD 20892, USA
* To whom correspondence should be addressed. Tel: +1 301 496 6068; Fax: +1 301 496 5239; Email: anndean{at}helix.nih.gov
Received July 28, 2004; Revised and Accepted August 30, 2004
We studied the mechanism by which an insulator interrupts enhancer signaling to a gene using stably replicated chromatin templates containing the human ß-globin locus control region HS2 enhancer and a target globin gene. The chicken ß-globin 5' HS4 (cHS4) insulator acted as a positional enhancer blocker, inhibiting promoter remodeling and transcription activation only when placed between the enhancer and gene. Enhancer blocking by cHS4 reduced histone hyperacetylation across a zone extending from the enhancer to the gene and inhibited recruitment of CBP and p300 to HS2. Enhancer blocking also led to accumulation of RNA polymerase II at HS2 and within cHS4, accompanied by its diminution at the gene promoter. The enhancer blocking effects were completely attributable to the CTCF binding site in cHS4. These findings provide experimental evidence for the involvement of spreading in establishment of a broad zone of histone modification by an enhancer, as well as for blocking by an insulator of the transfer of RNA polymerase II from an enhancer to a promoter.
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