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Nucleic Acids Research 2004 32(17):e134; doi:10.1093/nar/gnh129
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Published online 27 September 2004

Nucleic Acids Research, Vol. 32 No. 17 © Oxford University Press 2004; all rights reserved

Gene silencing in alveolar type II cells using cell-specific promoter in vitro and in vivo

Deming Gou, Telugu Narasaraju, Narendranath Reddy Chintagari, Nili Jin, Pengcheng Wang and Lin Liu*

Department of Physiological Sciences, Oklahoma State University, 264 McElroy Hall, Stillwater OK 74078, USA

* To whom correspondence should be addressed. Tel: +1 405 744 4526; Fax: +1 405 744 8263; Email: liulin{at}okstate.edu
The authors wish it to be known that, in their opinion, the first two authors should be regarded as joint First Authors

Received May 12, 2004; Revised July 27, 2004; Accepted August 20, 2004

RNA interference (RNAi) is a sequence-specific post-transcriptional gene silencing process. Although it is widely used in the loss-of-function studies, none of the current RNAi technologies can achieve cell-specific gene silencing. The lack of cell specificity limits its usage in vivo. Here, we report a cell-specific RNAi system using an alveolar epithelial type II cell-specific promoter—the surfactant protein C (SP-C) promoter. We show that the SP-C-driven small hairpin RNAs specifically depress the expression of the exogenous reporter (enhanced green fluorescent protein) and endogenous genes (lamin A/C and annexin A2) in alveolar type II cells, but not other lung cells, using cell and organ culture in vitro as well as in vivo. The present study provides an efficient strategy in silencing a gene in one type of cell without interfering with other cell systems, and may have a significant impact on RNAi therapy.


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