Published online 14 October 2004
Nucleic Acids Research, Vol. 32 No. 18 © Oxford University Press 2004; all rights reserved
DNA repair by a Rad22–Mus81-dependent pathway that is independent of Rhp51
Department of Biochemistry, University of Oxford, South Parks Road, Oxford OX1 3QU, UK
* To whom correspondence should be addressed. Tel: +44 1865 275192; Fax: +44 1865 275297; Email: matthew.whitby{at}bioch.ox.ac.uk
The authors wish it to be known that, in their opinion, the first two authors should be regarded as joint First Authors
Received June 8, 2004; Revised and Accepted September 9, 2004
In budding yeast most Rad51-dependent and -independent recombination depends on Rad52. In contrast, its homologue in fission yeast, Rad22, was assumed to play a less critical role possibly due to functional redundancy with another Rad52-like protein Rti1. We show here that this is not the case. Rad22 like Rad52 plays a central role in recombination being required for both Rhp51-dependent and -independent events. Having established this we proceed to investigate the involvement of the Mus81–Eme1 endonuclease in these pathways. Mus81 plays a relatively minor role in the Rhp51-dependent repair of DNA damage induced by ultraviolet light. In contrast Mus81 has a key role in the Rad22-dependent (Rhp51-independent) repair of damage induced by camptothecin, hydroxyurea and methyl-methanesulfonate. Furthermore, spontaneous intrachromosomal recombination that gives rise to deletion recombinants is impaired in a mus81 mutant. From these data we propose that a Rad22–Mus81-dependent (Rhp51-independent) pathway is an important mechanism for the repair of DNA damage in fission yeast. Consistent with this we show that in vitro Rad22 can promote strand invasion to form a D-loop that can be cleaved by Mus81.
CiteULike 
Connotea 
Del.icio.us What's this?
This article has been cited by other articles:
|
|
 |
|
  A. Lorenz, F. Osman, V. Folkyte, S. Sofueva, and M. C. Whitby Fbh1 Limits Rad51-Dependent Recombination at Blocked Replication Forks Mol. Cell. Biol., September 1, 2009; 29(17): 4742 - 4756. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 N. Willis and N. Rhind Mus81, Rhp51(Rad51), and Rqh1 Form an Epistatic Pathway Required for the S-Phase DNA Damage Checkpoint Mol. Biol. Cell, February 1, 2009; 20(3): 819 - 833. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. F. Pinter, S. D. Aubert, and V. A. Zakian The Schizosaccharomyces pombe Pfh1p DNA Helicase Is Essential for the Maintenance of Nuclear and Mitochondrial DNA Mol. Cell. Biol., November 1, 2008; 28(21): 6594 - 6608. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 W. Kagawa, A. Kagawa, K. Saito, S. Ikawa, T. Shibata, H. Kurumizaka, and S. Yokoyama Identification of a Second DNA Binding Site in the Human Rad52 Protein J. Biol. Chem., August 29, 2008; 283(35): 24264 - 24273. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 G. Octobre, A. Lorenz, J. Loidl, and J. Kohli The Rad52 Homologs Rad22 and Rti1 of Schizosaccharomyces pombe Are Not Essential for Meiotic Interhomolog Recombination, but Are Required for Meiotic Intrachromosomal Recombination and Mating-Type-Related DNA Repair Genetics, April 1, 2008; 178(4): 2399 - 2412. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B. Froget, J. Blaisonneau, S. Lambert, and G. Baldacci Cleavage of Stalled Forks by Fission Yeast Mus81/Eme1 in Absence of DNA Replication Checkpoint Mol. Biol. Cell, February 1, 2008; 19(2): 445 - 456. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. K. Cullen, S. P. Hussey, C. Walker, J. Prudden, B.-Y. Wee, A. Dave, J. S. Findlay, A. P. Savory, and T. C. Humphrey Break-Induced Loss of Heterozygosity in Fission Yeast: Dual Roles for Homologous Recombination in Promoting Translocations and Preventing De Novo Telomere Addition Mol. Cell. Biol., November 1, 2007; 27(21): 7745 - 7757. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Kosoy, T. M. Calonge, E. A. Outwin, and M. J. O'Connell Fission Yeast Rnf4 Homologs Are Required for DNA Repair J. Biol. Chem., July 13, 2007; 282(28): 20388 - 20394. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Decottignies Microhomology-Mediated End Joining in Fission Yeast Is Repressed by Pku70 and Relies on Genes Involved in Homologous Recombination Genetics, July 1, 2007; 176(3): 1403 - 1415. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Coulon, E. Noguchi, C. Noguchi, L.-L. Du, T. M. Nakamura, and P. Russell Rad22Rad52-dependent Repair of Ribosomal DNA Repeats Cleaved by Slx1-Slx4 Endonuclease Mol. Biol. Cell, April 1, 2006; 17(4): 2081 - 2090. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. Hiyama, M. Katsura, T. Yoshihara, M. Ishida, A. Kinomura, T. Tonda, T. Asahara, and K. Miyagawa Haploinsufficiency of the Mus81-Eme1 endonuclease activates the intra-S-phase and G2/M checkpoints and promotes rereplication in human cells Nucleic Acids Res., February 2, 2006; 34(3): 880 - 892. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
F. Osman, J. Dixon, A. R. Barr, and M. C. Whitby The F-Box DNA Helicase Fbh1 Prevents Rhp51-Dependent Recombination without Mediator Proteins Mol. Cell. Biol., September 15, 2005; 25(18): 8084 - 8096. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Kai, M. N. Boddy, P. Russell, and T. S.-F. Wang Replication checkpoint kinase Cds1 regulates Mus81 to preserve genome integrity during replication stress Genes & Dev., April 15, 2005; 19(8): 919 - 932. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. A. Roberts and M. F. White An Archaeal Endonuclease Displays Key Properties of Both Eukaryal XPF-ERCC1 and Mus81 J. Biol. Chem., February 18, 2005; 280(7): 5924 - 5928. [Abstract] [Full Text] [PDF]
|
|