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Nucleic Acids Research 2004 32(19):5851-5860; doi:10.1093/nar/gkh927
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Published online 1 November 2004

Nucleic Acids Research, Vol. 32 No. 19 © Oxford University Press 2004; all rights reserved

CTD kinase I is involved in RNA polymerase I transcription

Céline Bouchoux, Guillaume Hautbergue, Sabrina Grenetier, Christophe Carles, Michel Riva and Valérie Goguel*

Service de Biochimie et Génétique Moléculaire, CEA/Saclay, 91191 Gif/Yvette, France

* To whom correspondence should be addressed. Tel: +33 1 6908 9921; Fax: +33 1 6908 4712; Email: vgoguel{at}cea.fr
Present address: Guillaume Hautbergue, Department of Biology and Biotechnology, The University of Sheffield, Firth Court, Western Bank, Sheffield S10 2TN, UK

Received August 11, 2004; Revised September 17, 2004; Accepted October 20, 2004

RNA polymerase II carboxy terminal domain (CTD) kinases are key elements in the control of mRNA synthesis. Yeast CTD kinase I (CTDK-I), is a non-essential complex involved in the regulation of mRNA synthesis at the level of transcription elongation, pre-mRNA 3' formation and nuclear export. Here, we report that CTDK-I is also involved in ribosomal RNA synthesis. We show that CTDK-I is localized in part in the nucleolus. In its absence, nucleolar structure and RNA polymerase I transcription are affected. In vitro experiments show an impairment of the Pol I transcription machinery. Remarkably, RNA polymerase I co-precipitates from cellular extracts with Ctk1, the kinase subunit of the CTDK-I complex. In vitro analysis further demonstrates a direct interaction between RNA polymerase I and Ctk1. The results suggest that CTDK-I might participate in the regulation of distinct nuclear transcriptional machineries, thus playing a role in the adaptation of the global transcriptional response to growth signalling.


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