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Nucleic Acids Research 2004 32(19):5907-5915; doi:10.1093/nar/gkh920
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Published online 4 November 2004

Nucleic Acids Research, Vol. 32 No. 19 © Oxford University Press 2004; all rights reserved

Centromere parC of plasmid R1 is curved

Christian Hoischen, Alexander Bolshoy1, Kenn Gerdes2 and Stephan Diekmann*

Institute for Molecular Biotechnology e.V., Beutenbergstr. 11, D-07745 Jena, Germany, 1 Institute of Evolution, University of Haifa, Haifa 31905, Israel and 2 Department of Biochemistry and Molecular Biology, University of Southern Denmark, Campusvej 55, DK-5230 Odense M, Denmark

* To whom correspondence should be addressed. Tel: +49 3641 656 260; Fax: +49 3641 656 261; Email: diekmann{at}imb-jena.de

Received August 18, 2004; Revised and Accepted October 14, 2004

The centromere sequence parC of Escherichia coli low-copy-number plasmid R1 consists of two sets of 11 bp iterated sequences. Here we analysed the intrinsic sequence-directed curvature of parC by its migration anomaly in polyacrylamide gels. The 159 bp long parC is strongly curved with anomaly values (k-factors) close to 2. The properties of the parC curvature agree with those of other curved DNA sequences. parC contains two regions of 5-fold repeated iterons separated by 39 bp. We modified 4 bp within this intermediate sequence so that we could analyse the two 5-fold repeated regions independently. The analysis shows that the two repeat regions are not independently curved parts of parC but that the overall curvature is a property of the whole fragment. Since the centromere sequence of an E.coli plasmid as well as eukaryotic centromere sequences show DNA curvature, we speculate that curvature might be a general property of centromeres.


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