Skip Navigation

This Article
Right arrow Full Text Freely available
Right arrow Print PDF (93K) Freely available
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Search for citing articles in:
ISI Web of Science (13)
Right arrowRequest Permissions
Right arrow Commercial Re-use Guidelines
for Open Access NAR Content
Google Scholar
Right arrow Articles by Tork, S.
Right arrow Articles by Fabret, C.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Tork, S.
Right arrow Articles by Fabret, C.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Published online 21 January 2004

Nucleic Acids Research, 2004, Vol. 32, No. 2 415-421
© 2004 Oxford University Press

The major 5' determinant in stop codon read-through involves two adjacent adenines

Sanaa Tork, Isabelle Hatin, Jean-Pierre Rousset* and Céline Fabret

CNRS UMR 8621, Institut de Génétique et Microbiologie, Université Paris-Sud, 91405 Orsay Cedex, France

*To whom correspondence should be addressed. Tel: +33 169 155051; Fax: +33 169 154629; Email: rousset{at}igmors.u-psud.fr

The aim of this approach was to identify the major determinants, located at the 5' end of the stop codon, that modulate translational read-through in Saccharomyces cerevisiae. We developed a library of oligonucleotides degenerate at the six positions immediately upstream of the termination codon, cloned in the ADE2 reporter gene. Variations at these positions modulated translational read-through efficiency ~16-fold. The major effect was imposed by the two nucleotides immediately upstream of the stop codon. We showed that this effect was neither mediated by the last amino acid residues present in the polypeptide chain nor by the tRNA present in the ribosomal P site. We propose that the mRNA structure, depending on the nucleotides in the P site, is the main 5' determinant of read-through efficiency.


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 Nucleic Acids ResHome page
K. Sivaraman, A. Seshasayee, P. M. Tarwater, and A. M. Cole
Codon choice in genes depends on flanking sequence information--implications for theoretical reverse translation
Nucleic Acids Res., February 11, 2008; 36(3): e16 - e16.
[Abstract] [Full Text] [PDF]

Home page
 GeneticsHome page
I. Hatin, C. Fabret, O. Namy, W. A. Decatur, and J.-P. Rousset
Fine-Tuning of Translation Termination Efficiency in Saccharomyces cerevisiae Involves Two Factors in Close Proximity to the Exit Tunnel of the Ribosome
Genetics, November 1, 2007; 177(3): 1527 - 1537.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
A. G. Cridge, L. L. Major, A. A. Mahagaonkar, E. S. Poole, L. A. Isaksson, and W. P. Tate
Comparison of characteristics and function of translation termination signals between and within prokaryotic and eukaryotic organisms
Nucleic Acids Res., April 13, 2006; 34(7): 1959 - 1973.
[Abstract] [Full Text] [PDF]

Home page
 BioinformaticsHome page
M. Bhasin, A. Garg, and G. P. S. Raghava
PSLpred: prediction of subcellular localization of bacterial proteins
Bioinformatics, May 15, 2005; 21(10): 2522 - 2524.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
A. Garg, M. Bhasin, and G. P. S. Raghava
Support Vector Machine-based Method for Subcellular Localization of Human Proteins Using Amino Acid Compositions, Their Order, and Similarity Search
J. Biol. Chem., April 15, 2005; 280(15): 14427 - 14432.
[Abstract] [Full Text] [PDF]

Home page
 GeneticsHome page
M. K. Shirra, S. E. Rogers, D. E. Alexander, and K. M. Arndt
The Snf1 Protein Kinase and Sit4 Protein Phosphatase Have Opposing Functions in Regulating TATA-Binding Protein Association With the Saccharomyces cerevisiae INO1 Promoter
Genetics, April 1, 2005; 169(4): 1957 - 1972.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. Biol.Home page
S. Bai, B. He, and E. M. Wilson
Melanoma Antigen Gene Protein MAGE-11 Regulates Androgen Receptor Function by Modulating the Interdomain Interaction
Mol. Cell. Biol., February 15, 2005; 25(4): 1238 - 1257.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
I. Williams, J. Richardson, A. Starkey, and I. Stansfield
Genome-wide prediction of stop codon readthrough during translation in the yeast Saccharomyces cerevisiae
Nucleic Acids Res., December 15, 2004; 32(22): 6605 - 6616.
[Abstract] [Full Text] [PDF]


Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.