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Published online 29 January 2004

Nucleic Acids Research, 2004, Vol. 32, No. 2 623-631
© 2004 Oxford University Press

Linker phosphoramidite reagents for the attachment of the first nucleoside to underivatized solid-phase supports

Richard T. Pon* and Shuyuan Yu

Department of Biochemistry and Molecular Biology, University of Calgary, 3550 Hospital Drive N.W., Calgary, Alberta, Canada T2N 4N1

*To whom correspondence should be addressed. Tel: +1 403 220 4225; Email: rtpon{at}ucalgary.ca

New linker phosphoramidite reagents containing a cleavable 3'-ester linkage are used for attaching the first nucleoside to the surface of a solid- phase support. Inexpensive, underivatized amino supports, such as long chain alkylamine controlled-pore glass, can serve as universal supports. No modifications to phosphoramidite coupling conditions are required and, after synthesis, treatment with NH4OH releases the products with 3'-OH ends. No 3'-dephosphorylation is required. Phosphoramidite reagents containing a succinate and sulfonyl diethanol linkage between the nucleoside and phosphoramidite group are particularly advantageous and can be used to create both 3'-OH and 5'-phosphate ends on oligonucleotides. Reproducibility and quality of oligonucleotide synthesis is demonstrated for either column and 96-well plate formats on low-, medium- or high-loading CPG supports.


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R. T. Pon and S. Yu
Tandem oligonucleotide synthesis using linker phosphoramidites
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