Published online 29 January 2004
Nucleic Acids Research, 2004, Vol. 32, No. 2 e22
© 2004 Oxford University Press
Oligonucleotide-directed site-specific integration of high complexity libraries into ssDNA templates
1 Department of Molecular Pharmacology, 2 Department of Microbiology and Immunology and 3 Baxter Laboratory in Genetic Pharmacology, School of Medicine, Stanford University, Stanford, CA 94305, USA
*To whom correspondence should be addressed. Tel: +1 650 725 7002; Fax: +1 650 725 2383; Email: gnolan{at}stanford.edu
We present an approach that generates an oligomer-based library with minimal need for restriction site modification of sequences in the target vector. The technique has the advantage that it can be applied for generating peptide aptamer libraries at sites within proteins without the need for introducing flanking enzyme sites. As an example we present a phagemid retroviral shuttle vector that can be used to achieve stable expression of the library in mammalian cells for the purpose of screening for peptides with desired biological activity.