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Nucleic Acids Research 2004 32(20):6001-6014; doi:10.1093/nar/gkh923
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Published online 16 November 2004

Nucleic Acids Research, Vol. 32 No. 20 © Oxford University Press 2004; all rights reserved

Identification of a novel human nuclear-encoded mitochondrial poly(A) polymerase

Rafal Tomecki1, Aleksandra Dmochowska1,2, Kamil Gewartowski1,2, Andrzej Dziembowski1,2 and Piotr P. Stepien1,2,*

1 Department of Genetics, Warsaw University and 2 Institute of Biochemistry and Biophysics, Polish Academy of Sciences, Pawinskiego 5a, 02-106 Warsaw, Poland

* To whom correspondence should be addressed. Tel: +48 22 592 2240; Fax: +48 22 592 2244; Email: stepien{at}ibb.waw.pl

Received July 28, 2004; Revised and Accepted October 18, 2004

We report here on the identification of a novel human nuclear-encoded mitochondrial poly(A) polymerase. Immunocytochemical experiments confirm that the enzyme indeed localizes to mitochondrial compartment. Inhibition of expression of the enzyme by RNA interference results in significant shortening of the poly(A) tails of the mitochondrial ND3, COX III and ATP 6/8 transcripts, suggesting that the investigated protein represents a bona fide mitochondrial poly(A) polymerase. This is in agreement with our sequencing data which show that poly(A) tails of several mitochondrial messengers are composed almost exclusively of adenosine residues. Moreover, the data presented here indicate that all analyzed mitochondrial transcripts with profoundly shortened poly(A) tails are relatively stable, which in turn argues against the direct role of long poly(A) extensions in the stabilization of human mitochondrial messengers.


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